Tissue-specific dysregulation of 11 beta-hydroxysteroid dehydrogenase type 1 in overweight/obese women with polycystic ovary syndrome compared with weight-matched controls

Alessandra Gambineri, Flaminia Fanelli, Federica Tomassoni, Alessandra Munarini, Uberto Pagotto, Ruth Andrew, Brian R Walker, Renato Pasquali

Research output: Contribution to journalArticlepeer-review

Abstract

Context: Abnormal cortisol metabolism in polycystic ovary syndrome (PCOS) has been invoked as a cause of secondary activation of the hypothalamic-pituitary-adrenal axis and hence androgen excess. However, this is based on urinary excretion of cortisol metabolites, which cannot detect tissue-specific changes in metabolism and may be confounded by obesity.

Objective: To assess cortisol clearance and whole-body and tissue-specific activities of 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1 (HSD11B1)) in PCOS.

Design: Case-control study.

Setting: Medical center.

Patients: A total of 20 overweight-obese unmedicated Caucasian women with PCOS, aged 18-45 years, and 20 Caucasian controls matched for age, BMI, body fat distribution, and HSD11B1 genotypes (rs846910 and rs12086634).

Main outcome measures: Cortisol metabolites were measured in 24 h urine. During steady-state 9,11,12,12-[H-2](4)-cortisol infusion, cortisol clearance was calculated and whole-body HSD11B1 activity was assessed as the rate of appearance of 9,12,12-H-2(3)-cortisol (d3-cortisol). Hepatic HSD11B1 activity was quantified as the generation of plasma cortisol following an oral dose of cortisone. Subcutaneous adipose HSD11B1 activity and HSD11B1 mRNA were measured, ex vivo, in biopsies.

Results: Urinary cortisol metabolite excretion, deuterated cortisol clearance, and the rate of appearance of d3-cortisol did not differ between patients with PCOS and controls. However, hepatic HSD11B1 conversion of oral cortisone to cortisol was impaired (P

Conclusions: Tissue-specific dysregulation of HSD11B1 is a feature of PCOS, over and above obesity, whereas increased clearance of cortisol may result from obesity rather than PCOS.

Original languageEnglish
Pages (from-to)47-57
Number of pages11
JournalEuropean Journal of Endocrinology
Volume171
Issue number1
DOIs
Publication statusPublished - Jul 2014

Keywords

  • 11-beta-Hydroxysteroid Dehydrogenase Type 1
  • Adolescent
  • Adult
  • Case-Control Studies
  • Female
  • Humans
  • Hydrocortisone
  • Middle Aged
  • Obesity
  • Overweight
  • Polycystic Ovary Syndrome
  • Young Adult

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