Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry

A Molnar, A Lovas, Z Banfalvi*, L Lakatos, Z Polgar, S Horvath

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The molecular basis of the differential expression of the GM7-type metallocarboxypeptidase inhibitor (MCPI) genes in tuberizing (StMCPI) and non-tuberizing Solanum species (SbMCPI) was investigated. It was shown that the StMCPI is encoded by a gene family in Solanum tuberosum (potato), but SbMCPI might be a single-copy gene in the non-tuberizing species Solanum brevidens. The StMCPI promoter shows evolutionary relatedness to the S. brevidens-derived SbMCPI and to the fruit-specific tomato promoter 2A11. Both StMCPI and SbMCPI promoter regions were able to confer tuber- and berry-specific expression for the beta -glucuronidase reporter gene in potato suggesting that the difference in MCPI gene expression is in trans regulatory factors between the tuberizing and the non-tuberizing Solanum species. The MCPI promoters did not respond to metabolic, environmental or hormonal signals in leaves. Thus, the MCPI genes are regulated in a different way than the other known tuber-specific genes and potentially are suitable for biotechnological application in potato to provide specific transgene expression in tuber and berry.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalPlant molecular biology
Volume46
Issue number3
Publication statusPublished - Jun 2001

Keywords

  • berry
  • metallocarboxypeptidase inhibitor
  • organ-specific gene expression
  • potato
  • tomato
  • tuber
  • FRUIT-SPECIFIC EXPRESSION
  • SOLANUM-TUBEROSUM
  • STORAGE PROTEIN
  • DIFFERENTIAL EXPRESSION
  • SUCROSE SYNTHASE
  • TOMATO
  • PLANTS
  • STARCH
  • IDENTIFICATION
  • ACCUMULATION

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