Top-down protein sequencing by CID and ECD using desorption electrospray ionisation (DESI) and high-field FTICR mass spectrometry

Adam A. Stokes, David J. Clarke, Stefan Weidt, Pat Langridge-Smith, C. Logan Mackay

Research output: Contribution to journalArticlepeer-review

Abstract

We report high resolution spectra for the medium molecular weight proteins myoglobin and cytochrome-obtained using a custom desorption electrospray ionisation (DESI) source coupled to a Bruker Daltonics 12T Apex Qe Fourier transform ion cyclotron resonance mass spectrometer (FTICR-MS). The DESI source was designed for accurate alignment and reproduction of critical geometric variables. A two axis motorised stage was included to enable automated rastering of the sample under the DESI plume. Spectra for the intact proteins have been obtained under single-acquisition conditions and a top-down analysis of cytochrome-c was performed using both collision induced dissociation (CID) and electron capture dissociation (ECD) of the isolated [M+15H](15+) charge state. The sequence coverage is comparable to that obtained using electrospray ionisation, demonstrating the utility of top-down protein analysis by DESI FTICR-MS. (C) 2009 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)54-57
Number of pages4
JournalInternational Journal of Mass Spectrometry
Volume289
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010

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