Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces

Joaquin Seras-Franzoso, Penelope M. Tsimbouri, Karl V. Burgess, Ugutz Unzueta, Elena Garcia-Fruitos, Esther Vazquez, Antonio Villaverde, Matthew J. Dalby*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Bacterial inclusion bodies (IBs) are nanostructured (submicron), pseudospherical proteinaceous particles produced in recombinant bacteria resulting from ordered protein aggregation. Being mechanically stable, several physicochemical and biological properties of IBs can be tuned by appropriate selection of the producer strain and of culture conditions. It has been previously shown that IBs favor cell adhesion and surface colonization by mammalian cell lines upon decoration on materials surfaces, but how these biomaterials could influence the behavior of mesenchymal stem cells remains to be explored. Materials & methods: Here, the authors vary topography, stiffness and wettability using the IBs to decorate polycaprolactone surfaces on which mesenchymal stem cells are cultured. Results: The authors show that these topographies can be used to specifically target osteogenesis from mesenchymal stem cells, and through metabolomics, they show that the cells have increased energy demand during this bone-related differentiation. Conclusion: IBs as topographies can be used not only to direct cell proliferation but also to target differentiation of mesenchymal stem cells.

Original languageEnglish
Pages (from-to)207-220
Number of pages14
JournalNanomedicine
Volume9
Issue number2
DOIs
Publication statusPublished - 20 Feb 2014

Keywords

  • inclusion body
  • mesenchymal stem cell
  • metabolomic
  • topography

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