Toward universal forward genetics: Using a draft genome sequence of the nematode oscheius tipulae to identify mutations affecting vulva development

Fabrice Besnard*, Georgios Koutsovoulos, Sana Dieudonné, Mark Blaxter, Marie Anne Félix

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mapping-by-sequencing has become a standard method to map and identify phenotype-causing mutations in model species. Here, we show that a fragmented draft assembly is sufficient to perform mapping-by-sequencing in nonmodel species. We generated a draft assembly and annotation of the genome of the free-living nematode Oscheius tipulae, a distant relative of the model Caenorhabditis elegans. We used this draft to identify the likely causative mutations at the O. tipulae cov-3 locus, which affect vulval development. The cov-3 locus encodes the O. tipulae ortholog of C. elegans mig-13, and we further show that Cel-mig-13 mutants also have an unsuspected vulval-development phenotype. In a virtuous circle, we were able to use the linkage information collected during mutant mapping to improve the genome assembly. These results showcase the promise of genome-enabled forward genetics in nonmodel species.

Original languageEnglish
Pages (from-to)1747-1761
Number of pages15
JournalGenetics
Volume206
Issue number4
DOIs
Publication statusPublished - 1 Aug 2017

Keywords

  • Genome assembly
  • Mapping-by-sequencing
  • Mig-13
  • Oscheius tipulae
  • Vulva development

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