Towards combinatorial transcriptional engineering

Rajesh Mehrotra*, Kaushik Renganaath, Harsh Kanodia, Gary Loake, Sandhya Mehrotra

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The modular nature of the transcriptional unit makes it pos sible to design robust modules with predictable input -output characteristics using a ‘parts - off a shelf’ approach. Customized regulator y circuits composed of multiple such transcriptional units have immense scope for application in diverse fields of basic and applied research. Synthetic transcriptional engineering seeks to construct such genetic cascades. Here, we discuss the three principle strands of transcriptional engineering: promoter and transcriptional factor engineering, and programming inducibilty into synthetic modules. In this context, we review the scope and limitations of some recent technologies that seek to achieve these ends. Our discussion emphasizes a requirement for rational combinatorial engineering principles and the promise this approach holds for the future development of this field.
Original languageEnglish
Pages (from-to)390-405
Number of pages16
JournalBiotechnology Advances
Volume35
Issue number3
Early online date11 Mar 2017
DOIs
Publication statusPublished - 1 May 2017

Keywords

  • CRISPR
  • Gene expression
  • Promoters
  • Synthetic biology
  • Tale
  • Transcription factors
  • Transcriptional engineering

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