Towards combinatorial transcriptional engineering

Rajesh Mehrotra; Kaushik Renganaath; Harsh Kanodia; Gary Loake; Sandhya Mehrotra

doi:10.1016/j.biotechadv.2017.03.006

Towards combinatorial transcriptional engineering

Rajesh Mehrotra^*, Kaushik Renganaath, Harsh Kanodia, Gary Loake, Sandhya Mehrotra

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The modular nature of the transcriptional unit makes it pos sible to design robust modules with predictable input -output characteristics using a ‘parts - off a shelf’ approach. Customized regulator y circuits composed of multiple such transcriptional units have immense scope for application in diverse fields of basic and applied research. Synthetic transcriptional engineering seeks to construct such genetic cascades. Here, we discuss the three principle strands of transcriptional engineering: promoter and transcriptional factor engineering, and programming inducibilty into synthetic modules. In this context, we review the scope and limitations of some recent technologies that seek to achieve these ends. Our discussion emphasizes a requirement for rational combinatorial engineering principles and the promise this approach holds for the future development of this field.

Original language	English
Pages (from-to)	390-405
Number of pages	16
Journal	Biotechnology Advances
Volume	35
Issue number	3
Early online date	11 Mar 2017
DOIs	https://doi.org/10.1016/j.biotechadv.2017.03.006
Publication status	Published - 1 May 2017

Keywords / Materials (for Non-textual outputs)

CRISPR
Gene expression
Promoters
Synthetic biology
Tale
Transcription factors
Transcriptional engineering

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10.1016/j.biotechadv.2017.03.006

Towards combinatorial transcriptional engineering
© 2017 Elsevier Inc. All rights reserved
Accepted author manuscript, 0.99 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Cite this

@article{13af4a19c89740e5bd9e5cb70ee19115,

title = "Towards combinatorial transcriptional engineering",

abstract = "The modular nature of the transcriptional unit makes it pos sible to design robust modules with predictable input -output characteristics using a {\textquoteleft}parts - off a shelf{\textquoteright} approach. Customized regulator y circuits composed of multiple such transcriptional units have immense scope for application in diverse fields of basic and applied research. Synthetic transcriptional engineering seeks to construct such genetic cascades. Here, we discuss the three principle strands of transcriptional engineering: promoter and transcriptional factor engineering, and programming inducibilty into synthetic modules. In this context, we review the scope and limitations of some recent technologies that seek to achieve these ends. Our discussion emphasizes a requirement for rational combinatorial engineering principles and the promise this approach holds for the future development of this field.",

keywords = "CRISPR, Gene expression, Promoters, Synthetic biology, Tale, Transcription factors, Transcriptional engineering",

author = "Rajesh Mehrotra and Kaushik Renganaath and Harsh Kanodia and Gary Loake and Sandhya Mehrotra",

note = "Research in the Loake lab is supported by the BBSRC. RM is thankful to Indian National Science academy and Royal society of Edinburgh for support as bilateral exchange fellow to visit Loake lab.RM and SM are thankful to Department of Biological Sciences, BITS, Pilani for infrastructure support.",

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doi = "10.1016/j.biotechadv.2017.03.006",

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AU - Mehrotra, Rajesh

AU - Renganaath, Kaushik

AU - Kanodia, Harsh

AU - Loake, Gary

AU - Mehrotra, Sandhya

N1 - Research in the Loake lab is supported by the BBSRC. RM is thankful to Indian National Science academy and Royal society of Edinburgh for support as bilateral exchange fellow to visit Loake lab.RM and SM are thankful to Department of Biological Sciences, BITS, Pilani for infrastructure support.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - The modular nature of the transcriptional unit makes it pos sible to design robust modules with predictable input -output characteristics using a ‘parts - off a shelf’ approach. Customized regulator y circuits composed of multiple such transcriptional units have immense scope for application in diverse fields of basic and applied research. Synthetic transcriptional engineering seeks to construct such genetic cascades. Here, we discuss the three principle strands of transcriptional engineering: promoter and transcriptional factor engineering, and programming inducibilty into synthetic modules. In this context, we review the scope and limitations of some recent technologies that seek to achieve these ends. Our discussion emphasizes a requirement for rational combinatorial engineering principles and the promise this approach holds for the future development of this field.

AB - The modular nature of the transcriptional unit makes it pos sible to design robust modules with predictable input -output characteristics using a ‘parts - off a shelf’ approach. Customized regulator y circuits composed of multiple such transcriptional units have immense scope for application in diverse fields of basic and applied research. Synthetic transcriptional engineering seeks to construct such genetic cascades. Here, we discuss the three principle strands of transcriptional engineering: promoter and transcriptional factor engineering, and programming inducibilty into synthetic modules. In this context, we review the scope and limitations of some recent technologies that seek to achieve these ends. Our discussion emphasizes a requirement for rational combinatorial engineering principles and the promise this approach holds for the future development of this field.

KW - CRISPR

KW - Gene expression

KW - Promoters

KW - Synthetic biology

KW - Tale

KW - Transcription factors

KW - Transcriptional engineering

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DO - 10.1016/j.biotechadv.2017.03.006

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VL - 35

SP - 390

EP - 405

JO - Biotechnology Advances

JF - Biotechnology Advances

IS - 3

ER -

Towards combinatorial transcriptional engineering

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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