TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever

Jerome Nyhalah Dinga; Mark Wamalwa; Dieudonne Lemuh Njimoh; Moses N. Njahira; Appolinaire Djikeng; Rob Skilton; Vincent Pryde Kehdingha Titanji; Roger Pelle

doi:10.1371/journal.pone.0128040

TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever

Jerome Nyhalah Dinga, Mark Wamalwa, Dieudonne Lemuh Njimoh, Moses N. Njahira, Appolinaire Djikeng, Rob Skilton, Vincent Pryde Kehdingha Titanji^*, Roger Pelle

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction

East Coast fever, a devastating disease of cattle, can be controlled partially by vaccination with live T. parva sporozoites. The antigens responsible for conferring immunity are not fully characterized. Recently it was shown that the Plasmodium falciparum immunodominant protein UB05 is highly conserved in T. parva, the causative agent of East Coast fever. The aim of the present investigation was to determine the role of the homologue TpUB05 in protective immunity to East Coast fever.

Methods

The cloning, sequencing and expression of TpUB05 were done according to standard protocols. Bioinformatics analysis of TpUB05 gene was carried out using algorithms found in the public domain. Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. The ability of recombinant TpUB05 (r-TpUB05) to stimulate bovine PBMCs ex-vivo to produce IFN-gamma or to proliferate was tested using ELISpot and [3H]-thymidine incorporation assays, respectively.

Results

All the 20 cattle immunised by the infection and treatment method (ITM) developed significantly higher levels of TpUB05 specific antibodies (p

Conclusion

It was concluded that TpUB05 is a potential marker of protective immunity in ECF worth investigating further.

Original language	English
Article number	0128040
Number of pages	20
Journal	PLoS ONE
Volume	10
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0128040
Publication status	Published - 8 Jun 2015

Keywords / Materials (for Non-textual outputs)

SPOROZOITES
PREDICTION
ANTIBODIES
ANTIGENS

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Dinga, J. N., Wamalwa, M., Njimoh, D. L., Njahira, M. N., Djikeng, A., Skilton, R., Titanji, V. P. K., & Pelle, R. (2015). TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever. PLoS ONE, 10(6), Article 0128040. https://doi.org/10.1371/journal.pone.0128040

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title = "TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever",

abstract = "IntroductionEast Coast fever, a devastating disease of cattle, can be controlled partially by vaccination with live T. parva sporozoites. The antigens responsible for conferring immunity are not fully characterized. Recently it was shown that the Plasmodium falciparum immunodominant protein UB05 is highly conserved in T. parva, the causative agent of East Coast fever. The aim of the present investigation was to determine the role of the homologue TpUB05 in protective immunity to East Coast fever.MethodsThe cloning, sequencing and expression of TpUB05 were done according to standard protocols. Bioinformatics analysis of TpUB05 gene was carried out using algorithms found in the public domain. Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. The ability of recombinant TpUB05 (r-TpUB05) to stimulate bovine PBMCs ex-vivo to produce IFN-gamma or to proliferate was tested using ELISpot and [3H]-thymidine incorporation assays, respectively.ResultsAll the 20 cattle immunised by the infection and treatment method (ITM) developed significantly higher levels of TpUB05 specific antibodies (pConclusionIt was concluded that TpUB05 is a potential marker of protective immunity in ECF worth investigating further.",

keywords = "SPOROZOITES, PREDICTION, ANTIBODIES, ANTIGENS",

author = "Dinga, {Jerome Nyhalah} and Mark Wamalwa and Njimoh, {Dieudonne Lemuh} and Njahira, {Moses N.} and Appolinaire Djikeng and Rob Skilton and Titanji, {Vincent Pryde Kehdingha} and Roger Pelle",

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doi = "10.1371/journal.pone.0128040",

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TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever. / Dinga, Jerome Nyhalah; Wamalwa, Mark; Njimoh, Dieudonne Lemuh et al.
In: PLoS ONE, Vol. 10, No. 6, 0128040, 08.06.2015.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever

AU - Dinga, Jerome Nyhalah

AU - Wamalwa, Mark

AU - Njimoh, Dieudonne Lemuh

AU - Njahira, Moses N.

AU - Djikeng, Appolinaire

AU - Skilton, Rob

AU - Titanji, Vincent Pryde Kehdingha

AU - Pelle, Roger

PY - 2015/6/8

Y1 - 2015/6/8

N2 - IntroductionEast Coast fever, a devastating disease of cattle, can be controlled partially by vaccination with live T. parva sporozoites. The antigens responsible for conferring immunity are not fully characterized. Recently it was shown that the Plasmodium falciparum immunodominant protein UB05 is highly conserved in T. parva, the causative agent of East Coast fever. The aim of the present investigation was to determine the role of the homologue TpUB05 in protective immunity to East Coast fever.MethodsThe cloning, sequencing and expression of TpUB05 were done according to standard protocols. Bioinformatics analysis of TpUB05 gene was carried out using algorithms found in the public domain. Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. The ability of recombinant TpUB05 (r-TpUB05) to stimulate bovine PBMCs ex-vivo to produce IFN-gamma or to proliferate was tested using ELISpot and [3H]-thymidine incorporation assays, respectively.ResultsAll the 20 cattle immunised by the infection and treatment method (ITM) developed significantly higher levels of TpUB05 specific antibodies (pConclusionIt was concluded that TpUB05 is a potential marker of protective immunity in ECF worth investigating further.

AB - IntroductionEast Coast fever, a devastating disease of cattle, can be controlled partially by vaccination with live T. parva sporozoites. The antigens responsible for conferring immunity are not fully characterized. Recently it was shown that the Plasmodium falciparum immunodominant protein UB05 is highly conserved in T. parva, the causative agent of East Coast fever. The aim of the present investigation was to determine the role of the homologue TpUB05 in protective immunity to East Coast fever.MethodsThe cloning, sequencing and expression of TpUB05 were done according to standard protocols. Bioinformatics analysis of TpUB05 gene was carried out using algorithms found in the public domain. Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. The ability of recombinant TpUB05 (r-TpUB05) to stimulate bovine PBMCs ex-vivo to produce IFN-gamma or to proliferate was tested using ELISpot and [3H]-thymidine incorporation assays, respectively.ResultsAll the 20 cattle immunised by the infection and treatment method (ITM) developed significantly higher levels of TpUB05 specific antibodies (pConclusionIt was concluded that TpUB05 is a potential marker of protective immunity in ECF worth investigating further.

KW - SPOROZOITES

KW - PREDICTION

KW - ANTIBODIES

KW - ANTIGENS

U2 - 10.1371/journal.pone.0128040

DO - 10.1371/journal.pone.0128040

M3 - Article

SN - 1932-6203

VL - 10

JO - PLoS ONE

JF - PLoS ONE

IS - 6

M1 - 0128040

ER -

TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever

Abstract

Keywords / Materials (for Non-textual outputs)

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