Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence

John O. Mason*, Gareth T. Williams, Michael S. Neuberger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Immunoglobulin heavy chain gene transcription was studied using DNA transfection. The enhancer element identified in the mouse heavy chain locus was active in pre-B and plasmacytoma cell lines, but no activity was detected in two T cell lymphomas. However, even in the absence of the enhancer, cell type specificity of immunoglobulin gene transcription was still retained. We have used gene fusions to show that transcription cell type specificity is conferred by a VH gene promoter. Deletion analysis of this VH promoter indicates that a conserved octamer found 5′ of the TATA box in immunoglobulin V genes is a functional part of the tissue-specific promoter upstream element.

Original languageEnglish
Pages (from-to)479-487
Number of pages9
JournalCell
Volume41
Issue number2
DOIs
Publication statusPublished - Jun 1985

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