Transcription factor activator protein-2beta accelerates lipid accumulation in macrophages via enhancing the transcription of CD36

Lu Gan, Dongxing Zhu, Zhenhua Ding

Research output: Contribution to journalArticlepeer-review

Abstract

The role of transcription factor activator protein-2beta (AP-2beta) in foam cell formation was investigated. Uptake assay of DiI-labeled oxidized low density lipoprotein and the lipid quantitative analysis by high performance liquid chromatography showed AP-2beta promoted the lipid accumulation. The quantitative real-time RT-PCR and Western blot indicated that AP-2beta upregulated the expression of CD36. Luciferase assay, electrophoretic mobility shift assay and chromatin immunoprecipitation confirmed that AP-2beta bound to AP-2 binding sites in CD36 promoter region and enhanced the promoter activity of CD36 gene. We conclude that AP-2beta may function in foam cell formation through enhancing the transcription and expression of CD36 followed by increased lipid uptake.
Original languageEnglish
Pages (from-to)3615-9
Number of pages5
JournalFEBS Letters
Volume584
Issue number16
DOIs
Publication statusPublished - 20 Aug 2010

Keywords

  • Animals
  • Antigens, CD36
  • Atherosclerosis
  • Base Sequence
  • Binding Sites
  • Cell Line
  • DNA
  • Foam Cells
  • Humans
  • Lipid Metabolism
  • Lipoproteins, LDL
  • Macrophages
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Promoter Regions, Genetic
  • RNA, Small Interfering
  • Recombinant Proteins
  • Sequence Homology, Nucleic Acid
  • Transcription Factor AP-2
  • Transcription, Genetic

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