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Abstract / Description of output
The aim of this study was to elucidate transcriptional changes in the parasitic nematode Teladorsagia circumcincta upon encountering either naïve or immune ovine hosts. Pools of 100 000 exsheathed 3rd- stage T. circumcincta larvae were exposed in vitro to either an immune or naïve ovine abomasal environment, RNA was extracted from the larvae and sequenced using the Roche 454 platform. Each sample produced approximately 82 000 reads that assembled to give approximately 5500 Isotigs (contigs). The two sequence datasets were clustered together to give a total of 6969 clusters of which 18 were differentially expressed (P
Original language | English |
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Pages (from-to) | 387-405 |
Number of pages | 19 |
Journal | Parasitology |
Volume | 139 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2012 |
Keywords / Materials (for Non-textual outputs)
- Abomasum
- Adaptive Immunity
- Animals
- Gene Expression Profiling
- Gene Expression Regulation
- Helminth Proteins
- Host-Parasite Interactions
- Larva
- Proteomics
- RNA, Messenger
- Sequence Analysis, DNA
- Sheep
- Sheep Diseases
- Sheep, Domestic
- Tissue Extracts
- Trichostrongyloidea
- Trichostrongyloidiasis
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