Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status

Aileen M Halliday, F Alex Lainson, Raja Yaga, Neil F Inglis, Stephen Bridgett, Mintu Nath, Dave P Knox

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to elucidate transcriptional changes in the parasitic nematode Teladorsagia circumcincta upon encountering either naïve or immune ovine hosts. Pools of 100 000 exsheathed 3rd- stage T. circumcincta larvae were exposed in vitro to either an immune or naïve ovine abomasal environment, RNA was extracted from the larvae and sequenced using the Roche 454 platform. Each sample produced approximately 82 000 reads that assembled to give approximately 5500 Isotigs (contigs). The two sequence datasets were clustered together to give a total of 6969 clusters of which 18 were differentially expressed (P
Original languageEnglish
Pages (from-to)387-405
Number of pages19
JournalParasitology
Volume139
Issue number3
DOIs
Publication statusPublished - Mar 2012

Keywords

  • Abomasum
  • Adaptive Immunity
  • Animals
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Helminth Proteins
  • Host-Parasite Interactions
  • Larva
  • Proteomics
  • RNA, Messenger
  • Sequence Analysis, DNA
  • Sheep
  • Sheep Diseases
  • Sheep, Domestic
  • Tissue Extracts
  • Trichostrongyloidea
  • Trichostrongyloidiasis

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