Transcriptional expression levels of chicken collectins are affected by avian influenza A virus inoculation

Sylvia S. Reemers, Edwin J. A. Veldhuizen, Cherina Fleming, Daphne A. van Haarlem, Henk Haagsman, Lonneke Vervelde*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mammalian collect-ins have been found to play an important role in the defense against influenza A virus H9N2 inoculation, but for chicken collectins this has not yet been clarified. The aim of this study was to determine the effect of avian influenza A virus (AIV) inoculation on collectin gene expression in the respiratory tract of chickens and whether this was affected by age For this purpose 1- and 4-week-old chickens were inoculated intratracheally with PBS or H9N2 AIV Chickens were killed at 0, 8, 16 and 24 h post-inoculation and trachea and lung were harvested for analysis. Viral RNA expression and mRNA expression of chicken collectins 1 and 2 (cCL-1 and cCL-2), chicken lung lectin (cLL) and chicken surfactant protein A (cSP-A) were determined using real-time quantitative RT-PCR. In lung, a decrease in mRNA expression of cCL-2, cLL and cSP-A after inoculation with H9N2 was seen in both 1- and 4-week-old birds, although at different time points. while in trachea changes were only seen in 4-week-old birds and expression was increased Moreover. collectin expression correlated with viral RNA expression in lung of 1-week-old birds. These results suggest that both age and location in the respiratory tract affect changes in collectin mRNA expression after inoculation with H9N2 and indicate a possible role for collectins in the host response to AIV in the respiratory tract of chickens. (C) 2009 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalVeterinary Microbiology
Volume141
Issue number3-4
DOIs
Publication statusPublished - 24 Mar 2010

Keywords

  • BINDING
  • Avian influenza virus
  • GENE-EXPRESSION
  • C-type lectin
  • LUNG EPITHELIAL-CELLS
  • Innate immunity
  • LECTIN
  • SURFACTANT PROTEIN-D
  • RECOGNITION
  • INFECTION
  • Immune response
  • DOMAIN
  • CLONING

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