Transcriptional profiling of cecal gene expression in probiotic- and Salmonella-challenged neonatal chicks

S E Higgins, A D Wolfenden, G Tellez, B M Hargis, T E Porter

Research output: Contribution to journalArticlepeer-review


Probiotics are currently used to improve health and reduce enteric pathogens in poultry. However, the mechanisms by which they reduce or prevent disease are not known. Salmonella are intracellular pathogens that cause acute gastroenteritis in humans, and infections by nontyphoid species of Salmonella also can result in diarrhea, dehydration, and depression in poultry. Frequently, however, no clinical signs of infection are apparent in poultry flocks. In this study, day-of-hatch chicks were challenged with Salmonella enterica serovar Enteritidis (SE) and treated 1 h later with a poultry-derived, Lactobacillus-based probiotic culture (FloraMax-B11, Pacific Vet Group USA Inc., Fayetteville, AR). Cecae were collected 12 and 24 h posttreatment for Salmonella detection and RNA isolation for microarray analysis of gene expression. At both 12 and 24 h, SE was significantly reduced in chicks treated with the probiotic as compared with the birds challenged with only SE (P
Original languageEnglish
Pages (from-to)901-13
Number of pages13
JournalPoultry Science
Issue number4
Publication statusPublished - Apr 2011


  • Animals
  • Cecum
  • Chickens
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Humans
  • Lactobacillus
  • Oligonucleotide Array Sequence Analysis
  • Poultry Diseases
  • Probiotics
  • RNA, Bacterial
  • Random Allocation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salmonella Infections, Animal
  • Salmonella enteritidis

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