Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning

Filip J Wymeersch; Stavroula Skylaki; Yali Huang; Julia A Watson; Constantinos Economou; Carylyn Johnston; Simon R Tomlinson; Valerie Wilson

doi:10.1242/dev.168161

Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning

Filip J Wymeersch, Stavroula Skylaki, Yali Huang, Julia A Watson, Constantinos Economou, Carylyn Johnston, Simon R Tomlinson, Valerie Wilson

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The elongating mouse anteroposterior axis is supplied by progenitors with distinct tissue fates. It is not known whether these progenitors confer anteroposterior pattern to the embryo. We have analysed the progenitor population transcriptomes in the mouse primitive streak and tail bud throughout axial elongation. Transcriptomic signatures distinguish three known progenitor types (neuromesodermal, lateral/paraxial mesoderm and notochord progenitors; NMPs, LPMPs and NotoPs). Both NMP and LPMP transcriptomes change extensively over time. In particular, NMPs upregulate Wnt, Fgf, and Notch signalling components and many Hox genes as progenitors transit from production of the trunk to the tail and expand in number. In contrast, the transcriptome of NotoPs is stable throughout axial elongation and they are required for normal axis elongation. These results suggest that NotoPs act as a progenitor niche while anteroposterior patterning originates within NMPs and LPMPs.

Original language	English
Article number	168161
Pages (from-to)	1-16
Number of pages	16
Journal	Development
Volume	146
Issue number	1
Early online date	17 Dec 2018
DOIs	https://doi.org/10.1242/dev.168161
Publication status	Published - 2 Jan 2019

Keywords / Materials (for Non-textual outputs)

hox
lateral and paraxial mesoderm
mouse
neuromesodermal
notochord progenitors

Access to Document

10.1242/dev.168161Licence: Creative Commons: Attribution (CC-BY)

Transcriptionally dynamic PUB OA CC BY dev168161.full
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Final published version, 14.6 MBLicence: Creative Commons: Attribution (CC-BY)

Specification, maintenance and elimination of stem cell progenitors for the mammalian anteroposterior axis
Wilson, V.
MRC
1/01/13 → 31/12/18
Project: Research

Gene expression in microdissected embryonic regions during mouse axis elongation
Wymeersch, F. (Creator), Skylaki, S. (Creator), Huang, Y. (Creator), Watson, J. (Creator), Economou, C. (Creator), Marek-Johnston, C. (Creator), Tomlinson, S. (Creator) & Wilson, V. (Creator), National Center for Biotechnology Information (Gene Expression Omnibus), 6 Oct 2018
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120870
Dataset

Valerie Wilson
- v.wilsoned.acuk
- School of Biological Sciences - Personal Chair in Early Embryo Development
- Centre for Regenerative Medicine
Person: Academic: Research Active

Cite this

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title = "Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning",

abstract = "The elongating mouse anteroposterior axis is supplied by progenitors with distinct tissue fates. It is not known whether these progenitors confer anteroposterior pattern to the embryo. We have analysed the progenitor population transcriptomes in the mouse primitive streak and tail bud throughout axial elongation. Transcriptomic signatures distinguish three known progenitor types (neuromesodermal, lateral/paraxial mesoderm and notochord progenitors; NMPs, LPMPs and NotoPs). Both NMP and LPMP transcriptomes change extensively over time. In particular, NMPs upregulate Wnt, Fgf, and Notch signalling components and many Hox genes as progenitors transit from production of the trunk to the tail and expand in number. In contrast, the transcriptome of NotoPs is stable throughout axial elongation and they are required for normal axis elongation. These results suggest that NotoPs act as a progenitor niche while anteroposterior patterning originates within NMPs and LPMPs.",

keywords = "hox, lateral and paraxial mesoderm, mouse, neuromesodermal, notochord progenitors",

author = "Wymeersch, {Filip J} and Stavroula Skylaki and Yali Huang and Watson, {Julia A} and Constantinos Economou and Carylyn Johnston and Tomlinson, {Simon R} and Valerie Wilson",

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doi = "10.1242/dev.168161",

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AU - Wymeersch, Filip J

AU - Skylaki, Stavroula

AU - Huang, Yali

AU - Watson, Julia A

AU - Economou, Constantinos

AU - Johnston, Carylyn

AU - Tomlinson, Simon R

AU - Wilson, Valerie

PY - 2019/1/2

Y1 - 2019/1/2

N2 - The elongating mouse anteroposterior axis is supplied by progenitors with distinct tissue fates. It is not known whether these progenitors confer anteroposterior pattern to the embryo. We have analysed the progenitor population transcriptomes in the mouse primitive streak and tail bud throughout axial elongation. Transcriptomic signatures distinguish three known progenitor types (neuromesodermal, lateral/paraxial mesoderm and notochord progenitors; NMPs, LPMPs and NotoPs). Both NMP and LPMP transcriptomes change extensively over time. In particular, NMPs upregulate Wnt, Fgf, and Notch signalling components and many Hox genes as progenitors transit from production of the trunk to the tail and expand in number. In contrast, the transcriptome of NotoPs is stable throughout axial elongation and they are required for normal axis elongation. These results suggest that NotoPs act as a progenitor niche while anteroposterior patterning originates within NMPs and LPMPs.

AB - The elongating mouse anteroposterior axis is supplied by progenitors with distinct tissue fates. It is not known whether these progenitors confer anteroposterior pattern to the embryo. We have analysed the progenitor population transcriptomes in the mouse primitive streak and tail bud throughout axial elongation. Transcriptomic signatures distinguish three known progenitor types (neuromesodermal, lateral/paraxial mesoderm and notochord progenitors; NMPs, LPMPs and NotoPs). Both NMP and LPMP transcriptomes change extensively over time. In particular, NMPs upregulate Wnt, Fgf, and Notch signalling components and many Hox genes as progenitors transit from production of the trunk to the tail and expand in number. In contrast, the transcriptome of NotoPs is stable throughout axial elongation and they are required for normal axis elongation. These results suggest that NotoPs act as a progenitor niche while anteroposterior patterning originates within NMPs and LPMPs.

KW - hox

KW - lateral and paraxial mesoderm

KW - mouse

KW - neuromesodermal

KW - notochord progenitors

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DO - 10.1242/dev.168161

M3 - Article

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JF - Development

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Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

Specification, maintenance and elimination of stem cell progenitors for the mammalian anteroposterior axis

Datasets

Gene expression in microdissected embryonic regions during mouse axis elongation

Profiles

Valerie Wilson

Cite this