Transcriptome profile of murine gammaherpesvirus-68 lytic infection

Bahram Ebrahimi, Bernadette M Dutia, Kim L Roberts, Jose J Garcia-Ramirez, Paul Dickinson, James P Stewart, Peter Ghazal, Douglas J Roy, Anthony A Nash

Research output: Contribution to journalArticlepeer-review


The murine gammaherpesvirus-68 genome encodes 73 protein-coding open reading frames with extensive similarities to human gamma(2) herpesviruses, as well as unique genes and cellular homologues. We performed transcriptome analysis of stage-specific viral RNA during permissive infection using an oligonucleotide-based microarray. Using this approach, M4, K3, ORF38, ORF50, ORF57 and ORF73 were designated as immediate-early genes based on cycloheximide treatment. The microarray analysis also identified 10 transcripts with early expression kinetics, 32 transcripts with early-late expression kinetics and 29 transcripts with late expression kinetics. The latter group consisted mainly of structural proteins, and showed high expression levels relative to other viral transcripts. Moreover, we detected all eight tRNA-like transcripts in the presence of cycloheximide and phosphonoacetic acid. Lytic infection with MHV-68 also resulted in a significant reduction in the expression of cellular transcripts included in the DNA chip. This global approach to viral transcript analysis offers a powerful system for examining molecular transitions between lytic and latent virus infections associated with disease pathogenesis.
Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalJournal of General Virology
Issue numberPt 1
Publication statusPublished - 2003


  • Animals
  • Cells, Cultured
  • Cycloheximide
  • Enzyme Inhibitors
  • Epithelial Cells
  • Gammaherpesvirinae
  • Gene Expression Profiling
  • Herpesviridae Infections
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Open Reading Frames
  • Phosphonoacetic Acid
  • Protein Synthesis Inhibitors
  • Proteome
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic
  • Viral Proteins
  • Virus Latency


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