Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis

Manal Khalifé; Rachel Young; Bruno Passet; Sophie Halliez; Marthe Vilotte; Florence Jaffrezic; Sylvain Marthey; Vincent Béringue; Daniel Vaiman; Fabienne Le Provost; Hubert Laude; Jean-Luc Vilotte

doi:10.1371/journal.pone.0023253

Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis

Manal Khalifé, Rachel Young, Bruno Passet, Sophie Halliez, Marthe Vilotte, Florence Jaffrezic, Sylvain Marthey, Vincent Béringue, Daniel Vaiman, Fabienne Le Provost, Hubert Laude, Jean-Luc Vilotte

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.

Original language	English
Pages (from-to)	e23253
Journal	PLoS ONE
Volume	6
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0023253
Publication status	Published - 15 Aug 2011
Externally published	Yes

Keywords / Materials (for Non-textual outputs)

Animals
Embryo, Mammalian
Embryo, Nonmammalian
Embryonic Development
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Gene Regulatory Networks
Immunohistochemistry
Mice
Mice, Knockout
Prions
Protein Isoforms
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome
Zebrafish
Zebrafish Proteins

Access to Document

10.1371/journal.pone.0023253

Transcriptomic Analysis Brings New Insight into the Biological Role of the Prion Protein during Mouse Embryogenesis
©2011 Khalife et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 1.74 MBLicence: Creative Commons: Attribution (CC-BY)

Cite this

@article{fa45ec3d3598461c80688639befb4c36,

title = "Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis",

abstract = "The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.",

keywords = "Animals, Embryo, Mammalian, Embryo, Nonmammalian, Embryonic Development, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Gene Regulatory Networks, Immunohistochemistry, Mice, Mice, Knockout, Prions, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Transcriptome, Zebrafish, Zebrafish Proteins",

author = "Manal Khalif{\'e} and Rachel Young and Bruno Passet and Sophie Halliez and Marthe Vilotte and Florence Jaffrezic and Sylvain Marthey and Vincent B{\'e}ringue and Daniel Vaiman and {Le Provost}, Fabienne and Hubert Laude and Jean-Luc Vilotte",

year = "2011",

month = aug,

day = "15",

doi = "10.1371/journal.pone.0023253",

language = "English",

volume = "6",

pages = "e23253",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "8",

}

TY - JOUR

T1 - Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis

AU - Khalifé, Manal

AU - Young, Rachel

AU - Passet, Bruno

AU - Halliez, Sophie

AU - Vilotte, Marthe

AU - Jaffrezic, Florence

AU - Marthey, Sylvain

AU - Béringue, Vincent

AU - Vaiman, Daniel

AU - Le Provost, Fabienne

AU - Laude, Hubert

AU - Vilotte, Jean-Luc

PY - 2011/8/15

Y1 - 2011/8/15

N2 - The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.

AB - The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.

KW - Animals

KW - Embryo, Mammalian

KW - Embryo, Nonmammalian

KW - Embryonic Development

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Gene Knockdown Techniques

KW - Gene Regulatory Networks

KW - Immunohistochemistry

KW - Mice

KW - Mice, Knockout

KW - Prions

KW - Protein Isoforms

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Transcriptome

KW - Zebrafish

KW - Zebrafish Proteins

U2 - 10.1371/journal.pone.0023253

DO - 10.1371/journal.pone.0023253

M3 - Article

C2 - 21858045

SN - 1932-6203

VL - 6

SP - e23253

JO - PLoS ONE

JF - PLoS ONE

IS - 8

ER -

Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this