Transcriptomic analysis of the Chicken Anaemia Virus (CAV)-induced immunosuppression

E.S. Giotis; D.W. Burt; A.N.J. Scott; A. Downing; R.T. Talbot; L. Rothwell; P. Kaiser; E.J. Glass; D. Todd

doi:10.1016/j.vetimm.2008.10.073

Abstract

Chicken anaemia virus (CAV) is an unusually small virus that causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations, constituting a serious economic threat to poultry industry. The replication/pathogenicity of CAV relies on the expression of only a single structural protein VP1 and two non-structural proteins VP2 and VP3 (apoptin). The function of each individual protein is as yet unclear. This study has used transcriptional profiling to identify pathways that are dramatically modulated after 48 hr of infection in an in vitro model (MDCC-MSB1 cells) and after 4 days of experimental infection (1-day-old chickens in vivo model), using Affymetrix chicken oligonucleotide arrays. Changes in transcript levels between infected and uninfected cells indicate a dysfunction in the Mitogen Activated Protein kinases (MAPK) and T cell receptor signalling cascades implying that CAV might subvert host signalling responses to its own benefit. Two highly passaged attenuated cloned isolates and three VP3 CAV mutant viruses were also used in this study with the aim of elucidating the interactions of each of these proteins with the host cell. VP3 mutations resulted in lower levels of transcripts encoding signalling, transcription and mitosis related proteins. Overall, these data extend our understanding of how broadly CAV alters the regulation of host gene products and highlight the virus' 'need' to utilise the host cell machinery to replicate itself.

Original language	Undefined/Unknown
Pages	244
Number of pages	1
DOIs	https://doi.org/10.1016/j.vetimm.2008.10.073
Publication status	Published - 2009
Event	8th International Veterinary Immunology Symposium (8th IVIS) - Ouro Preto, Brazil Duration: 15 Aug 2007 → 19 Aug 2007

Conference

Conference	8th International Veterinary Immunology Symposium (8th IVIS)
Country/Territory	Brazil
City	Ouro Preto
Period	15/08/07 → 19/08/07

Keywords / Materials (for Non-textual outputs)

Chicken anaemia immunosuppression transcriptome

Access to Document

10.1016/j.vetimm.2008.10.073

ISP4 Pathogenesis and resistance in viral diseases of livestock
Dutia, B. (Principal Investigator), Archibald, A. (Co-investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Collie, D. (Co-investigator), Digard, P. (Co-investigator), Freeman, T. (Co-investigator), Glass, E. (Co-investigator), Grey, F. (Co-investigator), Hocking, P. (Co-investigator), Houston, R. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Nash, A. (Co-investigator), Sang, H. (Co-investigator), Sharp, C. (Co-investigator), Watson, M. (Co-investigator) & Whitelaw, B. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research
ARK-Genomics UK Centre for farm animal functional genomics 2
Burt, D. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/05/08 → 30/06/10
Project: Research

Cite this

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title = "Transcriptomic analysis of the Chicken Anaemia Virus (CAV)-induced immunosuppression",

abstract = "Chicken anaemia virus (CAV) is an unusually small virus that causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations, constituting a serious economic threat to poultry industry. The replication/pathogenicity of CAV relies on the expression of only a single structural protein VP1 and two non-structural proteins VP2 and VP3 (apoptin). The function of each individual protein is as yet unclear. This study has used transcriptional profiling to identify pathways that are dramatically modulated after 48 hr of infection in an in vitro model (MDCC-MSB1 cells) and after 4 days of experimental infection (1-day-old chickens in vivo model), using Affymetrix chicken oligonucleotide arrays. Changes in transcript levels between infected and uninfected cells indicate a dysfunction in the Mitogen Activated Protein kinases (MAPK) and T cell receptor signalling cascades implying that CAV might subvert host signalling responses to its own benefit. Two highly passaged attenuated cloned isolates and three VP3 CAV mutant viruses were also used in this study with the aim of elucidating the interactions of each of these proteins with the host cell. VP3 mutations resulted in lower levels of transcripts encoding signalling, transcription and mitosis related proteins. Overall, these data extend our understanding of how broadly CAV alters the regulation of host gene products and highlight the virus' 'need' to utilise the host cell machinery to replicate itself.",

keywords = "Chicken anaemia immunosuppression transcriptome",

author = "E.S. Giotis and D.W. Burt and A.N.J. Scott and A. Downing and R.T. Talbot and L. Rothwell and P. Kaiser and E.J. Glass and D. Todd",

note = "Transcriptomic analysis of the Chicken Anaemia Virus (CAV)-induced immunosuppression 0165-2427 RI: BBSRC Cat. C2, Conference poster abstract. Published (s4121) (Inst: ROSI) Prog. 20; Prog 1 Proj. 2800869 Author allocation: 1;1;3;1;1;2;2;1;3; 8th International Veterinary Immunology Symposium (8th IVIS) ; Conference date: 15-08-2007 Through 19-08-2007",

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AU - Giotis, E.S.

AU - Burt, D.W.

AU - Scott, A.N.J.

AU - Downing, A.

AU - Talbot, R.T.

AU - Rothwell, L.

AU - Kaiser, P.

AU - Glass, E.J.

AU - Todd, D.

N1 - Transcriptomic analysis of the Chicken Anaemia Virus (CAV)-induced immunosuppression 0165-2427 RI: BBSRC Cat. C2, Conference poster abstract. Published (s4121) (Inst: ROSI) Prog. 20; Prog 1 Proj. 2800869 Author allocation: 1;1;3;1;1;2;2;1;3

PY - 2009

Y1 - 2009

N2 - Chicken anaemia virus (CAV) is an unusually small virus that causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations, constituting a serious economic threat to poultry industry. The replication/pathogenicity of CAV relies on the expression of only a single structural protein VP1 and two non-structural proteins VP2 and VP3 (apoptin). The function of each individual protein is as yet unclear. This study has used transcriptional profiling to identify pathways that are dramatically modulated after 48 hr of infection in an in vitro model (MDCC-MSB1 cells) and after 4 days of experimental infection (1-day-old chickens in vivo model), using Affymetrix chicken oligonucleotide arrays. Changes in transcript levels between infected and uninfected cells indicate a dysfunction in the Mitogen Activated Protein kinases (MAPK) and T cell receptor signalling cascades implying that CAV might subvert host signalling responses to its own benefit. Two highly passaged attenuated cloned isolates and three VP3 CAV mutant viruses were also used in this study with the aim of elucidating the interactions of each of these proteins with the host cell. VP3 mutations resulted in lower levels of transcripts encoding signalling, transcription and mitosis related proteins. Overall, these data extend our understanding of how broadly CAV alters the regulation of host gene products and highlight the virus' 'need' to utilise the host cell machinery to replicate itself.

AB - Chicken anaemia virus (CAV) is an unusually small virus that causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations, constituting a serious economic threat to poultry industry. The replication/pathogenicity of CAV relies on the expression of only a single structural protein VP1 and two non-structural proteins VP2 and VP3 (apoptin). The function of each individual protein is as yet unclear. This study has used transcriptional profiling to identify pathways that are dramatically modulated after 48 hr of infection in an in vitro model (MDCC-MSB1 cells) and after 4 days of experimental infection (1-day-old chickens in vivo model), using Affymetrix chicken oligonucleotide arrays. Changes in transcript levels between infected and uninfected cells indicate a dysfunction in the Mitogen Activated Protein kinases (MAPK) and T cell receptor signalling cascades implying that CAV might subvert host signalling responses to its own benefit. Two highly passaged attenuated cloned isolates and three VP3 CAV mutant viruses were also used in this study with the aim of elucidating the interactions of each of these proteins with the host cell. VP3 mutations resulted in lower levels of transcripts encoding signalling, transcription and mitosis related proteins. Overall, these data extend our understanding of how broadly CAV alters the regulation of host gene products and highlight the virus' 'need' to utilise the host cell machinery to replicate itself.

KW - Chicken anaemia immunosuppression transcriptome

U2 - 10.1016/j.vetimm.2008.10.073

DO - 10.1016/j.vetimm.2008.10.073

M3 - Abstract

SP - 244

T2 - 8th International Veterinary Immunology Symposium (8th IVIS)

Y2 - 15 August 2007 through 19 August 2007

ER -

Transcriptomic analysis of the Chicken Anaemia Virus (CAV)-induced immunosuppression

Abstract

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

Projects

ISP4 Pathogenesis and resistance in viral diseases of livestock

ARK-Genomics UK Centre for farm animal functional genomics 2

Cite this