TY - JOUR
T1 - Transcytosis and surface presentation of IL-8 by venular endothelial cells
AU - Middleton, J.
AU - Neil, S.
AU - Clark-Lewis, I.
AU - Moore, H.
AU - Charles, L.
AU - Auer, M.
AU - Elin, H.
AU - Antal, R.
AU - Wintle, J.
N1 - Medline is the source for the MeSH terms of this document.
PY - 1997/10/31
Y1 - 1997/10/31
N2 - Chemokines have been convincingly implicated in actuating inflammatory leukocyte emigration. To affect the circulating leukocytes, tissue-derived chemokines have to traverse the endothelial cells (ECs). This was thought to be accomplished by chemokine diffusion through the intercellular gaps. On the contrary, we show by electron microscopy that the prototype chemokine IL-8 is internalized by venular ECs abluminally and transcytosed to the luminal surface. Here, it is presented to the adherent leukocytes on the EC membrane, predominantly in association with the EC projections. The intact C terminus of IL-8, the molecule's 'immobilization' domain, is required for the EC binding, transcytosis, and consequently, the in vivo proemigratory activity of IL-8, indicating that the described subcellular interactions of IL-8 with the ECs are functionally relevant.
AB - Chemokines have been convincingly implicated in actuating inflammatory leukocyte emigration. To affect the circulating leukocytes, tissue-derived chemokines have to traverse the endothelial cells (ECs). This was thought to be accomplished by chemokine diffusion through the intercellular gaps. On the contrary, we show by electron microscopy that the prototype chemokine IL-8 is internalized by venular ECs abluminally and transcytosed to the luminal surface. Here, it is presented to the adherent leukocytes on the EC membrane, predominantly in association with the EC projections. The intact C terminus of IL-8, the molecule's 'immobilization' domain, is required for the EC binding, transcytosis, and consequently, the in vivo proemigratory activity of IL-8, indicating that the described subcellular interactions of IL-8 with the ECs are functionally relevant.
UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-0030704740&md5=fdfe8b81f0a29f24f752860196e00c71
U2 - 10.1016/S0092-8674(00)80422-5
DO - 10.1016/S0092-8674(00)80422-5
M3 - Article
AN - SCOPUS:0030704740
SN - 0092-8674
VL - 91
SP - 385
EP - 395
JO - Cell
JF - Cell
IS - 3
ER -