Abstract

Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.

Original languageEnglish
Title of host publicationNMDA Receptors
Subtitle of host publicationMethods and Protocols
EditorsNail Burnashev, Pierre Szepetowski
Place of PublicationNew York, NY
PublisherHumana Press
Pages47-54
Number of pages8
Edition2
ISBN (Electronic)9781071638309
ISBN (Print)9781071638293, 9781071638323
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2799
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords / Materials (for Non-textual outputs)

  • Culture
  • Lipofection
  • Neuron
  • NMDA
  • Transfection

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