Transfection of rodent malaria parasites

Nisha Philip, Rachael Orr, Andrew P. Waters

Research output: Contribution to journalArticlepeer-review

Abstract

Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.

Original languageEnglish
Pages (from-to)99-125
Number of pages27
JournalMethods in Molecular Biology
Volume923
DOIs
Publication statusPublished - 25 Aug 2012

Keywords

  • animals
  • protozoan
  • cryopreservation
  • DNA
  • electroporation
  • female
  • flucytosine
  • humans
  • life cycle stages
  • malaria
  • mice
  • phenylhydrazines
  • plasmodium berghei
  • rodentia
  • Schizonts
  • transfection
  • genetically modified

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