Transgenic embryos and mice produced from low titre lentiviral vectors

Research output: Contribution to journalArticlepeer-review

Abstract

Lentiviral vectors are now recognised as an efficient transgene delivery system which can result in greater than 90% of founder animals carrying the transgene. Vector injection into the perivitelline space has emerged as the standard delivery method but is limited by the need for high-titre lentivirus vector preparations. Based on a modified perivitelline injection method we demonstrate that transgenic animals can be generated from low-titre virus vector preparations further simplifying lentiviral transgenesis. Repeat injection of 10(7) TU/ml vector preparation resulted in 23% of embryos carrying the transgene compare to 1% from a single injection. Embryos exposed to repeat injection of vector developed to blastocyst with the same efficiency as non-injected embryos and produced transgenic mice capable of transmitting the transgene through the germline.
Original languageEnglish
Pages (from-to)661-4
Number of pages4
JournalTransgenic Research
Volume16
Issue number5
DOIs
Publication statusPublished - Oct 2007

Keywords

  • Animals
  • Animals, Genetically Modified
  • Biotechnology
  • Gene Transfer Techniques
  • Genetic Techniques
  • Genetic Vectors
  • Lentivirus
  • Mice
  • Mice, Transgenic
  • Retroviridae
  • Transduction, Genetic
  • Transgenes

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