Transmission electron microscopy: Preparation of specimens

Bettina Boettcher

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract / Description of output

Sample preparation of biological specimens for electron microscopy aims at bringing the sample into a suitable size (<500 nm thick, accommodated on a 3 mm diameter, round sample carrier) and to strengthen these samples against the adverse conditions in the electron microscope (beam damage and vacuum). There are two types of samples, one are large cellular structures, which require further sectioning and the other are individual assemblies, which are small enough to be imaged in suspension. Any preparation method of these types of samples has to fulfil four basic requirements: (1) to avoid collapse of the structures in the vacuum of the electron microscope; (2) to provide a sample sufficiently thin to avoid multiple scattering of electrons; (3) to minimise structural alterations as a consequence of the damage by the electron beam; (4) to maximise the contrast in the resolution band of interest. The relative importance of each constraint varies with the type of specimen and with the level of resolution sought
Original languageEnglish
Title of host publicationWiley Online Library
PublisherJohn Wiley & Sons Inc.
Publication statusPublished - 15 Jun 2012

Keywords / Materials (for Non-textual outputs)

  • electron microscopy
  • sample preparation


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