Tuning fluorophore excitation in a total-internal-reflection-fluorescence microscopy

E. Sheykhi, B. Sajad, S. Tavaddod, H. Naderi-Manesh, N. Roostaiei

Research output: Contribution to journalArticlepeer-review

Abstract

In a total-internal-reflection-fluorescence-microscopy method, there is anisotropy in the polarized evanescentwave. Since the evanescent wave is used as an excitation field, the mentioned anisotropy is a disadvantage inusing the total-internal-reflection-fluorescence-microscopy technique. Therefore, by theoretical and analyticalapproaches, and based on the Fresnel coefficients, the effect of three dielectrics media on the anisotropy of theevanescent wave is investigated. Following that, a proper combination of the cover glass, oil immersion, and prismfor both living and non-living samples is suggested that not only enhances the intensity of the evanescent wave, butalso and importantly, decreases the essential anisotropy of the evanescent wave.
Original languageEnglish
Pages (from-to)8055-8060
Number of pages6
JournalApplied optics
Volume58
Issue number29
DOIs
Publication statusPublished - 9 Oct 2019

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