Projects per year
Abstract
We demonstrate a fast two-color widefield fluorescence microendoscopy system capable of simultaneously detecting several disease targets in intact human ex vivo lung tissue. We characterize the system for light throughput from the excitation light emitting diodes, fluorescence collection efficiency, and chromatic focal shifts. We demonstrate the effectiveness of the instrument by imaging bacteria (Pseudomonas aeruginosa) in ex vivo human lung tissue. We describe a mechanism of bacterial detection through the fiber bundle that uses blinking effects of bacteria as they move in front of the fiber core providing detection of objects smaller than the fiber core and cladding (∼3 μm∼3 μm). This effectively increases the measured spatial resolution of 4 μm4 μm. We show simultaneous imaging of neutrophils, monocytes, and fungus (Aspergillus fumigatus) in ex vivo human lung tissue. The instrument has 10 nM and 50 nM sensitivity for fluorescein and Cy5 solutions, respectively. Lung tissue autofluorescence remains visible at up to 200 fps camera acquisition rate. The optical system lends itself to clinical translation due to high-fluorescence sensitivity, simplicity, and the ability to multiplex several pathological molecular imaging targets simultaneously.
Original language | English |
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Article number | 046009 |
Journal | Journal of Biomedical Optics |
Volume | 21 |
Issue number | 4 |
DOIs | |
Publication status | Published - 27 Apr 2016 |
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Dive into the research topics of 'Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue'. Together they form a unique fingerprint.Projects
- 1 Finished
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Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M. (Principal Investigator)
1/10/13 → 31/03/19
Project: Research
Datasets
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Datasets for Journal of Biomedical Optics paper - "Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue"
Krstajic, N. (Creator), Akram, A.-U.-H. (Creator), Choudhary, T. (Creator), McDonald, N. (Creator), Pedretti, E. (Creator), Scholefield, E. (Creator) & Dhaliwal, K. (Creator), Edinburgh DataShare, 2016
DOI: 10.7488/ds/1372
Dataset
Profiles
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Kevin Dhaliwal
- Deanery of Clinical Sciences - Personal Chair of Molecular Imaging & Healthcare
- Centre for Inflammation Research
- Edinburgh Imaging
Person: Academic: Research Active , Academic: Research Active (Research Assistant)