Two-dimensional and three-dimensional viability measurements of adult stem cells with optical coherence phase microscopy

Pierre O. Bagnaninchi, Christina Holmes, Nicola Drummond, Jamal Daoud, Maryam Tabrizian

Research output: Contribution to journalArticlepeer-review

Abstract

Cell viability assays are essential tools for cell biology. They assess healthy cells in a sample and enable the quantification of cellular responses to reagents of interest. Noninvasive and label-free assays are desirable in two-dimensional (2D) and three-dimensional (3D) cell culture to facilitate time-course viability studies. Cellular micromotion, emanating from cell to substrate distance variations, has been demonstrated as a marker of cell viability with electric cell-substrate impedance sensing (ECIS). In this study we investigated if optical coherence phase microscopy (OCPM) was able to report phase fluctuations of adult stem cells in 2D and 3D that could be associated with cellular micromotion. An OCPM has been developed around a Thor labs engine (lambda o = 930 rim) and integrated in an inverted microscope with a custom scanning head. Human adipose derived stem cells (ADSCs, Invitrogen) were cultured in Mesenpro RS medium and seeded either on ECIS arrays, 2D cell culture dishes, or in 3D highly porous microplotted polymeric scaffolds. ADSC micromotion was confirmed by ECIS analysis. Live and fixed ADSCs were then investigated in 2D and 3D with OCPM. Significant differences were found in phase fluctuations between the different conditions. This study indicated that OCPM could potentially assess cell vitality in 2D and in 3D microstructures. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3606561]

Original languageEnglish
Article number086003
Pages (from-to)-
Number of pages8
JournalJournal of Biomedical Optics
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2011

Keywords

  • optical coherence phase microscopy
  • cell motility
  • three-dimensional scaffold
  • tissue engineering
  • phase fluctuations
  • DIGITAL HOLOGRAPHIC MICROSCOPY
  • LIVING CELLS
  • TISSUE
  • TOMOGRAPHY
  • SCAFFOLDS
  • DYNAMICS
  • FABRICATION
  • CULTURE

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