Projects per year
Triggering physiological responses with a light switch has become a reality with the development of smart molecular probes such as photolabile protecting groups (PPGs), able to “uncage” biological ligands on demand. To make the light switch virtually harmless and confine the excitation to the single-cell level, the caged ligands can be released using two-photon (2 P) absorption and 2 P microscopy using red/infrared light. This exceptional level of precision however comes at the cost of a reduced photosensitivity and a poor compatibility of early PPGs with 2 P excitation. This review aims to provide a tutorial guidebook to the design of 2 P-sensitive PPGs suitable for optobiology by discussing challenges, strategies and progress in uncaging of bioactive compounds. To do so, we first recall the photo-physical principles governing 2 P absorption, and the resulting ground rules in the design of efficient 2 P absorbing organic dyes. We then detail how following these guidelines has led to tremendous progress in the development of a new generation of caged compounds, and the implications in the fields of biophotonics, from neurology to targeted therapy.
|Journal||Journal of Photochemistry and Photobiology C: Photochemistry Reviews|
|Early online date||31 May 2021|
|Publication status||E-pub ahead of print - 31 May 2021|