Type V myosin focuses the polarisome and shapes the tip of yeast cells

Alexander Dünkler, Marcin Leda, Jan-Michael Kromer, Joachim Neller, Thomas Gronemeyer, Andrew B. Goryachev, Nils Johnsson

Research output: Contribution to journalArticlepeer-review


The polarisome is a cortical proteinaceous micro-compartment that organizes the growth of actin filaments and the fusion of secretory vesicles in yeasts and filamentous fungi. Polarisomes are compact, spot-like structures at the growing tips of their respective cells. The molecular forces that control form and size of this micro-compartment are not known. Here we identify a complex between the polarisome subunit Pea2 and the type V Myosin Myo2 that anchors Myo2 at the cortex of yeast cells. We discovered a point mutation in the cargo-binding domain of Myo2 that impairs the interaction with Pea2 and consequently the formation and focused localization of the polarisome. Cells carrying this mutation grow round instead of elongated buds. Further experiments and biophysical modeling suggest that the interactions between polarisome-bound Myo2 motors and dynamic actin filaments spatially focus the polarisome and sustain its compact shape.


Original languageEnglish
Article numbere202006193
Number of pages18
JournalJournal of Cell Biology
Issue number5
Early online date3 Mar 2021
Publication statusE-pub ahead of print - 3 Mar 2021


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