U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA

J P Morrissey, D Tollervey

Research output: Contribution to journalArticlepeer-review

Abstract

The small nucleolar RNA (snoRNA) U14 has two regions of extended primary sequence complementarity to the 18S rRNA. The 3' region (domain B) shows the consensus structure for the methylation guide class of snoRNAs, whereas base-pairing between the 5' region (domain A) and the 18S rRNA sequence is required for the formation of functional ribosomes. Between domains A and B lies another essential region (domain Y). Here we report that yeast U14 can be cross-linked in vivo to the pre-rRNA; cross-linking is detected exclusively with the 35S primary transcript. Many nucleotides in U14 that lie outside of domains A and B are cross-linked to the pre-rRNA; in particular the essential domain Y region is cross-linked at several sites. U14 is, therefore, in far more extensive contact with the pre-rRNA than predicted from simple base-pairing models. Moreover, U14 can be cross-linked to other small RNA species. The functional interactions made by U14 during ribosome synthesis are likely to be very complex.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalChromosoma
Volume105
Issue number7-8
DOIs
Publication statusPublished - Jun 1997

Keywords

  • psoralen
  • primer extension analysis
  • external transcribe space
  • functional ribosome
  • methylation guide

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