Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch

Jed Long, Thomas R. A. Gallagher, James R. Cavey, Paul W. Sheppard, Stuart H. Ralston, Robert Layfield, Mark S. Searle

Research output: Contribution to journalArticlepeer-review

Abstract

The p62 protein functions as a scaffold in signaling pathways that lead to activation of NF-kappa B and is an important regulator of osteoclastogenesis. Mutations affecting the receptor activator of NF-kappa B signaling axis can result in human skeletal disorders, including those identified in the C-terminal ubiquitin-associated (UBA) domain of p62 in patients with Paget disease of bone. These observations suggest that the disease may involve a common mechanism related to alterations in the ubiquitin-binding properties of p62. The structural basis for ubiquitin recognition by the UBA domain of p62 has been investigated using NMR and reveals a novel binding mechanism involving a slow exchange structural reorganization of the UBA domain to a "bound" non-canonical UBA conformation that is not significantly populated in the absence of ubiquitin. The repacking of the three-helix bundle generates a binding surface localized around the conserved Xaa-Gly-Phe-Xaa loop that appears to optimize both hydrophobic and electrostatic surface complementarity with ubiquitin. NMR titration analysis shows that the p62-UBA binds to Lys(48)-linked di-ubiquitin with similar to 4-fold lower affinity than to mono-ubiquitin, suggesting preferential binding of the p62-UBA to single ubiquitin units, consistent with the apparent in vivo preference of the p62 protein for Lys(63)-linked polyubiquitin chains (which adopt a more open and extended structure). The conformational switch observed on binding may represent a novel mechanism that underlies specificity in regulating signal-induced protein recognition events.

Original languageEnglish
Pages (from-to)5427-5440
Number of pages14
JournalJournal of Biological Chemistry
Volume283
Issue number9
DOIs
Publication statusPublished - 29 Feb 2008

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