Ultrastable shelled PFC nanobubbles: a platform for ultrasound-assisted diagnostics and therapy

Patrizia Nadia  Hanieh; Caterina  Ricci; Andrea  Bettucci; Roberto  Marotta; Carmel M Moran; Laura  Cantù; Maria  Carafa; Federica  Rinaldi; Elena  Del Favero; Carlotta  Marianecci

doi:10.1016/j.nano.2022.102611

Ultrastable shelled PFC nanobubbles: a platform for ultrasound-assisted diagnostics and therapy

Patrizia Nadia Hanieh, Caterina Ricci, Andrea Bettucci, Roberto Marotta, Carmel M Moran, Laura Cantù, Maria Carafa, Federica Rinaldi^*, Elena Del Favero^*, Carlotta Marianecci

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Nanoscale echogenic bubbles (NBs), can be used as a theranostic platform for the localized
delivery of encapsulated drugs. However, the generation of NBs is challenging, because they have
lifetimes as short as milliseconds in solution. The aim of this work has been the optimization of a
preparation method for the generation of stable NBs, characterized by measuring: a) acoustic
efficiency, b) nano-size, to ensure passive tumour targeting, c) stability during storage and after
injection d) ability to entrap drugs. NBs are monodisperse and ultra-stable, their stability achieved
by generation of an amphiphilic multilamellar shell able to efficiently retain the PFC gas. The NBs
perform as good acoustic enhancers over a wide frequency range and out of resonant conditions,
as tested in both in vitro and in vivo experiments, proving to be a potential platform for the
production of versatile carriers to be used in ultrasound-assisted diagnostic, therapeutic and
theranostic applications.

Original language	English
Journal	Nanomedicine: Nanotechnology, Biology and Medicine
Early online date	10 Oct 2022
DOIs	https://doi.org/10.1016/j.nano.2022.102611
Publication status	E-pub ahead of print - 10 Oct 2022

Keywords / Materials (for Non-textual outputs)

Nanobubbles
ultrasound contrast agents
acoustics
theranostics
Drug delivery

Access to Document

10.1016/j.nano.2022.102611

JN2022368Accepted author manuscript, 2.1 MBLicence: Creative Commons: Attribution (CC-BY)

Edinburgh Preclinical Imaging
Carmel Moran (Manager), Adrian Thomson (Manager), Ross J Lennen (Manager), Adriana Tavares (Manager), Carlos J. Alcaide-Corral (Manager), Tim Morgan (Other), Islay Cranston (Other) & Kerry O'Rourke (Other)
Deanery of Clinical Sciences
Facility/equipment: Facility

Cite this

@article{110de407e7de4b688ee392845a3f6ab1,

title = "Ultrastable shelled PFC nanobubbles: a platform for ultrasound-assisted diagnostics and therapy",

abstract = "Nanoscale echogenic bubbles (NBs), can be used as a theranostic platform for the localizeddelivery of encapsulated drugs. However, the generation of NBs is challenging, because they havelifetimes as short as milliseconds in solution. The aim of this work has been the optimization of apreparation method for the generation of stable NBs, characterized by measuring: a) acousticefficiency, b) nano-size, to ensure passive tumour targeting, c) stability during storage and afterinjection d) ability to entrap drugs. NBs are monodisperse and ultra-stable, their stability achievedby generation of an amphiphilic multilamellar shell able to efficiently retain the PFC gas. The NBsperform as good acoustic enhancers over a wide frequency range and out of resonant conditions,as tested in both in vitro and in vivo experiments, proving to be a potential platform for theproduction of versatile carriers to be used in ultrasound-assisted diagnostic, therapeutic andtheranostic applications.",

keywords = "Nanobubbles, ultrasound contrast agents, acoustics, theranostics, Drug delivery",

author = "Hanieh, {Patrizia Nadia} and Caterina Ricci and Andrea Bettucci and Roberto Marotta and Moran, {Carmel M} and Laura Cant{\`u} and Maria Carafa and Federica Rinaldi and {Del Favero}, Elena and Carlotta Marianecci",

note = "Funding Information: We thank ESRF and ID02 staff for beamtime and assistance (10.15151/ESRF-ES-281427365). E.D.F. thanks BIOMETRA Dept. for partial support (PSR2020_DEL_FAVERO). Publisher Copyright: {\textcopyright} 2022",

year = "2022",

month = oct,

day = "10",

doi = "10.1016/j.nano.2022.102611",

language = "English",

journal = "Nanomedicine: Nanotechnology, Biology and Medicine",

issn = "1549-9634",

publisher = "Elsevier",

}

TY - JOUR

T1 - Ultrastable shelled PFC nanobubbles

T2 - a platform for ultrasound-assisted diagnostics and therapy

AU - Hanieh, Patrizia Nadia

AU - Ricci, Caterina

AU - Bettucci, Andrea

AU - Marotta, Roberto

AU - Moran, Carmel M

AU - Cantù, Laura

AU - Carafa, Maria

AU - Rinaldi, Federica

AU - Del Favero, Elena

AU - Marianecci, Carlotta

N1 - Funding Information: We thank ESRF and ID02 staff for beamtime and assistance (10.15151/ESRF-ES-281427365). E.D.F. thanks BIOMETRA Dept. for partial support (PSR2020_DEL_FAVERO). Publisher Copyright: © 2022

PY - 2022/10/10

Y1 - 2022/10/10

N2 - Nanoscale echogenic bubbles (NBs), can be used as a theranostic platform for the localizeddelivery of encapsulated drugs. However, the generation of NBs is challenging, because they havelifetimes as short as milliseconds in solution. The aim of this work has been the optimization of apreparation method for the generation of stable NBs, characterized by measuring: a) acousticefficiency, b) nano-size, to ensure passive tumour targeting, c) stability during storage and afterinjection d) ability to entrap drugs. NBs are monodisperse and ultra-stable, their stability achievedby generation of an amphiphilic multilamellar shell able to efficiently retain the PFC gas. The NBsperform as good acoustic enhancers over a wide frequency range and out of resonant conditions,as tested in both in vitro and in vivo experiments, proving to be a potential platform for theproduction of versatile carriers to be used in ultrasound-assisted diagnostic, therapeutic andtheranostic applications.

AB - Nanoscale echogenic bubbles (NBs), can be used as a theranostic platform for the localizeddelivery of encapsulated drugs. However, the generation of NBs is challenging, because they havelifetimes as short as milliseconds in solution. The aim of this work has been the optimization of apreparation method for the generation of stable NBs, characterized by measuring: a) acousticefficiency, b) nano-size, to ensure passive tumour targeting, c) stability during storage and afterinjection d) ability to entrap drugs. NBs are monodisperse and ultra-stable, their stability achievedby generation of an amphiphilic multilamellar shell able to efficiently retain the PFC gas. The NBsperform as good acoustic enhancers over a wide frequency range and out of resonant conditions,as tested in both in vitro and in vivo experiments, proving to be a potential platform for theproduction of versatile carriers to be used in ultrasound-assisted diagnostic, therapeutic andtheranostic applications.

KW - Nanobubbles

KW - ultrasound contrast agents

KW - acoustics

KW - theranostics

KW - Drug delivery

U2 - 10.1016/j.nano.2022.102611

DO - 10.1016/j.nano.2022.102611

M3 - Article

SN - 1549-9634

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

ER -

Ultrastable shelled PFC nanobubbles: a platform for ultrasound-assisted diagnostics and therapy

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Edinburgh Preclinical Imaging

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