Ultraviolet irradiation increases FADD protein in apoptotic human keratinocytes

Peter K.M. Kim*, Richard Weller, Yun Hua, Timothy R. Billiar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Ultraviolet irradiation (UV) can induce keratinocyte apoptosis by activating death receptors that recruit the intracellular adaptor molecule FADD/MORT1 (Fas-associating death domain protein/mediator of receptor-induced toxicity). We hypothesized that UV could alter FADD expression levels to augment UV-induced keratinocyte apoptosis. In a dose-dependent manner UV B irradiation increased the expression of FADD protein in a human keratinocyte cell line (CCD-1106) with a corresponding increase in caspase-8 cleavage and cellular apoptosis. FADD overexpression induced cell death in 80% of cells compared with 10% spontaneous cell death in controls. Inhibition of FADD protein by adenoviral expression of anti-sense FADD reduced keratinocyte apoptosis. Regulation of FADD expression by UV may serve to enhance death receptor-mediated keratinocyte death.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number2
DOIs
Publication statusPublished - 10 Jan 2003

Keywords / Materials (for Non-textual outputs)

  • Apoptosis
  • FADD
  • Keratinocyte
  • MORT1
  • Ultraviolet irradiation

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