Understanding bovine trypanosomiasis and trypanotolerance: the promise of functional genomics

Emmeline W Hill, Grace M O'Gorman, Morris Agaba, John P Gibson, Olivier Hanotte, Stephen J Kemp, Jan Naessens, Paul M Coussens, David E MacHugh

Research output: Contribution to journalArticlepeer-review

Abstract

African bovine trypanosomiasis, caused by the protozoan parasite Trypanosoma congolense, is endemic throughout sub-Saharan Africa and is a major constraint on livestock production. A promising approach to disease control is to understand and exploit naturally evolved trypanotolerance. We describe the first attempt to investigate the transcriptional response of susceptible Boran (Bos indicus) cattle to trypanosome infection via a functional genomics approach using a bovine total leukocyte (BOTL) cDNA microarray platform. Four male Boran cattle were experimentally infected with T. congolense and peripheral blood mononuclear cells (PBMC) were collected before infection and 13, 17, 23 and 30 days post-infection (dpi). A reference experimental design was employed using a universal bovine reference RNA pool. Data were normalised to the median of a set of invariant genes (GAPDH) and BRB-Array tools was used to search for statistically significant differentially expressed genes between each time-point. Using a set of 20 microarray hybridisations, we have made a significant contribution to understand the temporal transcriptional response of bovine PBMC in vivo to a controlled trypanosome infection. The greatest changes were evident 13 dpi after parasites were first detected in the blood. Significant differences were observed in clusters of protein kinase C subunits and MHC class I/II related molecules.

Original languageEnglish
Pages (from-to)247-58
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume105
Issue number3-4
DOIs
Publication statusPublished - 15 May 2005

Keywords

  • Animals
  • Cattle
  • Disease Susceptibility
  • Expressed Sequence Tags
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genomics
  • Immunity, Innate
  • Leukocytes
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Parasitemia
  • Time Factors
  • Trypanosoma congolense
  • Trypanosomiasis, African
  • Trypanosomiasis, Bovine

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