Projects per year
Abstract / Description of output
Results: Salmon head kidney (SHK-1) cells were single-cell RNA sequenced at 24h, 48h and 96h post-ISAV challenge. At 24h post infection, cells showed expression signatures consistent with viral entry, with genes such as PI3K, FAK or JNK being upregulated relative to uninfected cells. At 48h and 96h, infected cells showed a clear anti-viral response, characterised by the expression of IFNA2 or IRF2. Uninfected bystander cells at 48h and 96h also showed clear transcriptional differences, potentially suggesting paracrine signalling from infected cells. These bystander cells expressed pathways such as mRNA sensing, RNA degradation, ubiquitination or proteasome; and up-regulation of mitochondrial ribosome genes also seemed to play a role in the host response to the infection. Correlation between viral and host genes revealed novel genes potentially key for this fish-virus interaction.
Conclusions: This study has increased our understanding of the cellular response of Atlantic salmon during ISAV infection and revealed host-virus interactions at the cellular level. Our results highlight various potential key genes in this host-virus interaction, which can be manipulated in future functional studies to increase the resistance of Atlantic salmon to ISAV.
Keywords / Materials (for Non-textual outputs)
- Salmo salar
- single cell RNA-Seq
FingerprintDive into the research topics of 'Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing'. Together they form a unique fingerprint.
- 3 Finished
Improving resistance to infectious salmon anaemia using genome editing: Novel approaches to tackling viral disease in aquaculture
1/04/18 → 31/03/22
1/10/12 → 30/09/14