Projects per year
To connect gene expression with cellular physiology, we need to follow levels of proteins over time. Experiments typically use variants of Green Fluorescent Protein (GFP), and time-series measurements require specialist expertise if single cells are to be followed. Fluorescence plate readers, however, a standard in many laboratories, can in principle provide similar data, albeit at a mean, population level. Nevertheless, extracting the average fluorescence per cell is challenging because autofluorescence can be substantial.
|Publication status||Published - 3 Feb 2014|
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- 1 Finished
A mathematical and experimental study of feedback in the single-cell dynamics of a transcriptional network in budding yeast
1/09/11 → 31/08/14