Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata

Tessa Moses; Jacob Pollier; Ahmad Faizal; Sandra Apers; Luc Pieters; Johan M Thevelein; Danny Geelen; Alain Goossens

doi:10.1016/j.molp.2014.11.004

Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata

Tessa Moses, Jacob Pollier, Ahmad Faizal, Sandra Apers, Luc Pieters, Johan M Thevelein, Danny Geelen, Alain Goossens

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.

Original language	English
Pages (from-to)	122-135
Number of pages	13
Journal	Molecular Plant
Volume	8
Issue number	1
Early online date	5 Jan 2015
DOIs	https://doi.org/10.1016/j.molp.2014.11.004
Publication status	E-pub ahead of print - 5 Jan 2015

Keywords / Materials (for Non-textual outputs)

cytochrome P450
oxidosqualene cyclase
C-28 oxidase
C-16α oxidase
triterpenoid saponin
maesa lanceolata

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10.1016/j.molp.2014.11.004

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title = "Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata",

abstract = "Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.",

keywords = "cytochrome P450, oxidosqualene cyclase, C-28 oxidase, C-16α oxidase, triterpenoid saponin, maesa lanceolata",

author = "Tessa Moses and Jacob Pollier and Ahmad Faizal and Sandra Apers and Luc Pieters and Thevelein, \{Johan M\} and Danny Geelen and Alain Goossens",

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doi = "10.1016/j.molp.2014.11.004",

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T1 - Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata

AU - Moses, Tessa

AU - Pollier, Jacob

AU - Faizal, Ahmad

AU - Apers, Sandra

AU - Pieters, Luc

AU - Thevelein, Johan M

AU - Geelen, Danny

AU - Goossens, Alain

PY - 2015/1/5

Y1 - 2015/1/5

N2 - Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.

AB - Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.

KW - cytochrome P450

KW - oxidosqualene cyclase

KW - C-28 oxidase

KW - C-16α oxidase

KW - triterpenoid saponin

KW - maesa lanceolata

U2 - 10.1016/j.molp.2014.11.004

DO - 10.1016/j.molp.2014.11.004

M3 - Article

C2 - 25578277

SN - 1674-2052

VL - 8

SP - 122

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JO - Molecular Plant

JF - Molecular Plant

IS - 1

ER -

Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata

Abstract

Keywords / Materials (for Non-textual outputs)

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