TY - JOUR
T1 - Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles
AU - Erdbrügger, Uta
AU - Blijdorp, Charles J.
AU - Bijnsdorp, Irene V
AU - Borràs, Francesc E
AU - Burger, Dylan
AU - Bussolati, Benedetta
AU - Byrd, James Brian
AU - Clayton, Aled
AU - Dear, James W
AU - Falcón-Pérez, Juan M.
AU - Grange, Cristina
AU - Hill, Andrew F.
AU - Holthöfer, Harry
AU - Hoorn, Ewout J.
AU - Jenster, Guido
AU - Jimenez, Connie R.
AU - Junker, Kerstin
AU - Klein, John
AU - Knepper, M A
AU - Koritzinsky, Erik H.
AU - Luther, James M.
AU - Lenassi, Metka
AU - Leivo, Janne
AU - Mertens, Inge
AU - Musante, Luca
AU - Oeyen, Eline
AU - Puhka, Maija
AU - van Royen, Martin E.
AU - Sánchez, Catherine
AU - Soekmadji, Carolina
AU - Thongboonkerd, Visith
AU - van Steijn, Volkert
AU - Verhaegh, Gerald
AU - Webber, Jason P.
AU - Witwer, Kenneth W.
AU - Yuen, P S T
AU - Lei, Zheng
AU - Llorente, Alicia
AU - Martens-Uzunova, Elena S.
PY - 2021/5/21
Y1 - 2021/5/21
N2 - Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
AB - Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
U2 - 10.1002/jev2.12093
DO - 10.1002/jev2.12093
M3 - Article
SN - 2001-3078
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
ER -