Use of a new immunoassay to measure PrP levels in scrapie-infected sheep brains reveals PrP genotype-specific differences

S. McCutcheon, F. Houston, N. Hunter

Research output: Contribution to journalArticlepeer-review

Abstract

The diagnosis of prion diseases, such as scrapie and BSE, has traditionally relied upon the identification of the disease-associated form of the prion protein, PrP, based on its resistance to digestion by proteinase K (PK). A more recent development is the conformation-dependent immunoassay (CDI), which distinguishes between PrP and normal PrP (PrP) based on their differing solubility in guanidine hydrochloride rather than resistance or sensitivity to PK. We have developed a CDI-formatted sandwich immunoassay for the measurement of PrP in sheep brain, which discriminates between clinically affected scrapie cases (natural or experimental) and uninfected controls of the same PrP genotype. Using this method, we have shown for the first time that, in sheep, the PrP genotype has a significant influence on the amount of PrP deposited in the brains of animals experimentally infected with scrapie.
Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalJournal of Immunological Methods
Volume298
Issue number1-2
DOIs
Publication statusPublished - 1 Mar 2005

Fingerprint

Dive into the research topics of 'Use of a new immunoassay to measure PrP levels in scrapie-infected sheep brains reveals PrP genotype-specific differences'. Together they form a unique fingerprint.

Cite this