Use of D140 conditional-knockout cell lines to study chromosomal passenger protein function

Xavier Fant, Kumilo Samejima, Ana Carvalho, Hiromi Ogawa, Zhenjie Xu, Zuojun Yue, William C. Earnshaw, Sandrine Ruchaud

Research output: Contribution to journalArticlepeer-review

Abstract

The CPC [chromosomal passenger complex INCENP (inner centromere protein) Aurora B kinase survivin and borealin] is implicated in many mitotic processes In the present paper we describe how we generated DT40 conditional knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity

Original languageEnglish
Pages (from-to)1655-1659
Number of pages5
JournalBiochemical Society Transactions
Volume38
DOIs
Publication statusPublished - Dec 2010

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