Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature

Campbell G Nicol; Laura Denby; Oscar Lopez-Franco; Rachel Masson; Crawford A Halliday; Stuart A Nicklin; Angelika Kritz; Lorraine M Work; Andrew H Baker

doi:10.1016/j.febslet.2009.05.037

Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature

Campbell G Nicol, Laura Denby, Oscar Lopez-Franco, Rachel Masson, Crawford A Halliday, Stuart A Nicklin, Angelika Kritz, Lorraine M Work, Andrew H Baker

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.

Original language	English
Pages (from-to)	2100-7
Number of pages	8
Journal	FEBS Letters
Volume	583
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2009.05.037
Publication status	Published - 18 Jun 2009

Keywords / Materials (for Non-textual outputs)

Adenoviridae
Amino Acid Sequence
Animals
Base Sequence
Cardiovascular Diseases
Coronary Vessels
DNA Primers
Disease Models, Animal
Endothelium, Vascular
Gene Expression
Genetic Engineering
Genetic Therapy
Genetic Vectors
Hepatocytes
Humans
Male
Oligopeptides
Peptide Library
Rats
Rats, Inbred SHR
Rats, Inbred WKY

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10.1016/j.febslet.2009.05.037

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@article{2ea74e13ebc742129fc945735f167552,

title = "Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature",

abstract = "We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.",

keywords = "Adenoviridae, Amino Acid Sequence, Animals, Base Sequence, Cardiovascular Diseases, Coronary Vessels, DNA Primers, Disease Models, Animal, Endothelium, Vascular, Gene Expression, Genetic Engineering, Genetic Therapy, Genetic Vectors, Hepatocytes, Humans, Male, Oligopeptides, Peptide Library, Rats, Rats, Inbred SHR, Rats, Inbred WKY",

author = "Nicol, {Campbell G} and Laura Denby and Oscar Lopez-Franco and Rachel Masson and Halliday, {Crawford A} and Nicklin, {Stuart A} and Angelika Kritz and Work, {Lorraine M} and Baker, {Andrew H}",

year = "2009",

month = jun,

day = "18",

doi = "10.1016/j.febslet.2009.05.037",

language = "English",

volume = "583",

pages = "2100--7",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature

AU - Nicol, Campbell G

AU - Denby, Laura

AU - Lopez-Franco, Oscar

AU - Masson, Rachel

AU - Halliday, Crawford A

AU - Nicklin, Stuart A

AU - Kritz, Angelika

AU - Work, Lorraine M

AU - Baker, Andrew H

PY - 2009/6/18

Y1 - 2009/6/18

N2 - We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.

AB - We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.

KW - Adenoviridae

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cardiovascular Diseases

KW - Coronary Vessels

KW - DNA Primers

KW - Disease Models, Animal

KW - Endothelium, Vascular

KW - Gene Expression

KW - Genetic Engineering

KW - Genetic Therapy

KW - Genetic Vectors

KW - Hepatocytes

KW - Humans

KW - Male

KW - Oligopeptides

KW - Peptide Library

KW - Rats

KW - Rats, Inbred SHR

KW - Rats, Inbred WKY

U2 - 10.1016/j.febslet.2009.05.037

DO - 10.1016/j.febslet.2009.05.037

M3 - Article

C2 - 19481546

SN - 0014-5793

VL - 583

SP - 2100

EP - 2107

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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