Use of time-resolved FRET to validate crystal structure of complement regulatory complex between C3b and factor H (N terminus)

Isabell C. Pechtl, Robert K. Neely, David T. F. Dryden, Anita C. Jones, Paul N. Barlow

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Structural knowledge of interactions amongst the similar to 40 proteins of the human complement system, which is central to immune surveillance and homeostasis, is expanding due primarily to X-ray diffraction of co-crystallized proteins. Orthogonal evidence, in solution, for the physiological relevance of such co-crystal structures is valuable since intermolecular affinities are generally weak-to-medium and inter-domain mobility may be important. In this current work, Forster resonance energy transfer (FRET) was used to investigate the 10 mu M K-D (210 kD) complex between the N-terminal region of the soluble complement regulator, factor H (FH1-4), and the key activation-specific complement fragment, C3b. Using site-directed mutagenesis, seven cysteines were introduced individually at potentially informative positions within the four CCP modules comprising FH1-4, then used for fluorophore attachment. C3b possesses a thioester domain featuring an internal cycloglutamyl cysteine thioester; upon hydrolysis this yields a free thiol (Cys988) that was also fluorescently tagged. Labeled proteins were functionally active as cofactors for cleavage of C3b to iC3b except for FH1-4(Q40C) where conjugation with the fluorophore likely abrogated interaction with the protease, factor I. Time-resolved FRET measurements were undertaken to explore interactions between FH1-4 and C3b in fluid phase and under near-physiological conditions. These experiments confirmed that, as in the cocrystal structure, FH1-4 binds to C3b with CCP module 1 furthest from, and CCP module 4 closest to, the thioester domain, placing subsequent modules of FH near to any surface to which C3b is attached. The data do not rule out flexibility of the thioester domain relative to the remainder of the complex.

Original languageEnglish
Pages (from-to)2102-2112
Number of pages11
JournalProtein Science
Issue number12
Publication statusPublished - Dec 2011

Keywords / Materials (for Non-textual outputs)

  • resonance energy transfer
  • time-resolved fluorescence
  • complement system
  • factor H
  • C3b
  • fluorescent protein labeling


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