Using human artificial chromosomes to study centromere assembly and function

Oscar Molina; Natalay Kouprina; Hiroshi Masumoto; Vladimir Larionov; William Earnshaw

doi:10.1007/s00412-017-0633-x

Using human artificial chromosomes to study centromere assembly and function

Oscar Molina, Natalay Kouprina, Hiroshi Masumoto, Vladimir Larionov, William Earnshaw

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using human artificial chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies.

Original language	English
Pages (from-to)	559-575
Number of pages	17
Journal	Chromosoma
Early online date	7 Jul 2017
DOIs	https://doi.org/10.1007/s00412-017-0633-x
Publication status	Published - 1 Oct 2017

Keywords / Materials (for Non-textual outputs)

human artificial chromosomes
centromere
kinetochore
CENP-A
mitosis

Access to Document

10.1007/s00412-017-0633-xLicence: All Rights Reserved

Using human artificial chromosomes to study centromere assembly and functionAccepted author manuscript, 1.5 MB

The role of non-histone proteins in chromosome structure and function during mitosis
Earnshaw, B.
UK-based charities
1/01/11 → 30/09/16
Project: Research

Cite this

@article{ebbba2243c0e481ea3b401e3c1e818f7,

title = "Using human artificial chromosomes to study centromere assembly and function",

abstract = "Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using human artificial chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies.",

keywords = "human artificial chromosomes, centromere, kinetochore, CENP-A, mitosis",

author = "Oscar Molina and Natalay Kouprina and Hiroshi Masumoto and Vladimir Larionov and William Earnshaw",

year = "2017",

month = oct,

day = "1",

doi = "10.1007/s00412-017-0633-x",

language = "English",

pages = "559--575",

journal = "Chromosoma",

issn = "0009-5915",

publisher = "Springer",

}

TY - JOUR

T1 - Using human artificial chromosomes to study centromere assembly and function

AU - Molina, Oscar

AU - Kouprina, Natalay

AU - Masumoto, Hiroshi

AU - Larionov, Vladimir

AU - Earnshaw, William

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using human artificial chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies.

AB - Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using human artificial chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies.

KW - human artificial chromosomes

KW - centromere

KW - kinetochore

KW - CENP-A

KW - mitosis

U2 - 10.1007/s00412-017-0633-x

DO - 10.1007/s00412-017-0633-x

M3 - Article

SN - 0009-5915

SP - 559

EP - 575

JO - Chromosoma

JF - Chromosoma

ER -

Using human artificial chromosomes to study centromere assembly and function

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

The role of non-histone proteins in chromosome structure and function during mitosis

Cite this