Using TraceFinder and Excel software to evaluate and report multi-analyte targeted LC-MS data acquired on an ThermoScientific Exploris 240 Orbitrap v1

In our lab we focus on targeted analysis and have found that acquiring LC-HRMS data using Xcalibur software and assessing the data using TraceFinder software is the most robust and flexible approach.
This protocol describes our approach to evaluating LC-HRMS data that has been collected in Xcalibur software, on LC-HRMS instruments including ThermoScientific Exploris mass spectrometers. It describes how to build a processing method in TraceFinder and using it to evaluate a full scan analysis data set acquired in Xcalibur software.
The LC-HRMS data set must contain a calibration curve and unknowns analysed as a batch using the same Acquisition method in Xcalibur. The results from TraceFinder are then transferred to Microsoft Excel to summarise the calculated amounts of the analytes of interest in the samples as a final result.