Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature

Grant D Stewart; Jan Baird; Frances Rae; Jyoti Nanda; Antony C P Riddick; David J Harrison

doi:10.1007/s11255-011-9948-3

Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature

Grant D Stewart, Jan Baird, Frances Rae, Jyoti Nanda, Antony C P Riddick, David J Harrison

Research output: Contribution to journal › Article › peer-review

Abstract

Translational prostate cancer research is hampered by long intervals from diagnosis to patient progression and difficulty in obtaining cancer tissue for investigation. As such, it is imperative to utilise aging formalin-fixed paraffin-embedded (FFPE) tissue samples from the pathology archive with linked patient outcome data to allow current day research. This study aimed to assess the adequacy and quantity of mRNA extracted from formalin fixed paraffin embedded (FFPE) prostate tissue, including prostate biopsies, up to 15 years old. The decay of mRNA over time and under differing storage conditions was also assessed.

Original language	English
Pages (from-to)	961-7
Number of pages	7
Journal	International urology and nephrology
Volume	43
Issue number	4
DOIs	https://doi.org/10.1007/s11255-011-9948-3
Publication status	Published - Dec 2011

Keywords / Materials (for Non-textual outputs)

Analysis of Variance
Biopsy
Formaldehyde
Humans
Male
Paraffin Embedding
Prostate
Prostatic Hyperplasia
RNA, Messenger
Real-Time Polymerase Chain Reaction
Specimen Handling
Temperature
Time Factors

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10.1007/s11255-011-9948-3

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Stewart, G. D., Baird, J., Rae, F., Nanda, J., Riddick, A. C. P., & Harrison, D. J. (2011). Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature. International urology and nephrology, 43(4), 961-7. https://doi.org/10.1007/s11255-011-9948-3

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title = "Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature",

abstract = "Translational prostate cancer research is hampered by long intervals from diagnosis to patient progression and difficulty in obtaining cancer tissue for investigation. As such, it is imperative to utilise aging formalin-fixed paraffin-embedded (FFPE) tissue samples from the pathology archive with linked patient outcome data to allow current day research. This study aimed to assess the adequacy and quantity of mRNA extracted from formalin fixed paraffin embedded (FFPE) prostate tissue, including prostate biopsies, up to 15 years old. The decay of mRNA over time and under differing storage conditions was also assessed.",

keywords = "Analysis of Variance, Biopsy, Formaldehyde, Humans, Male, Paraffin Embedding, Prostate, Prostatic Hyperplasia, RNA, Messenger, Real-Time Polymerase Chain Reaction, Specimen Handling, Temperature, Time Factors",

author = "Stewart, \{Grant D\} and Jan Baird and Frances Rae and Jyoti Nanda and Riddick, \{Antony C P\} and Harrison, \{David J\}",

year = "2011",

month = dec,

doi = "10.1007/s11255-011-9948-3",

language = "English",

volume = "43",

pages = "961--7",

journal = "International urology and nephrology",

issn = "1573-2584",

publisher = "Springer",

number = "4",

}

Stewart, GD, Baird, J, Rae, F, Nanda, J, Riddick, ACP & Harrison, DJ 2011, 'Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature', International urology and nephrology, vol. 43, no. 4, pp. 961-7. https://doi.org/10.1007/s11255-011-9948-3

Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature. / Stewart, Grant D; Baird, Jan; Rae, Frances et al.
In: International urology and nephrology, Vol. 43, No. 4, 12.2011, p. 961-7.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research

T2 - assessment of the effect of increasing sample age and storage temperature

AU - Stewart, Grant D

AU - Baird, Jan

AU - Rae, Frances

AU - Nanda, Jyoti

AU - Riddick, Antony C P

AU - Harrison, David J

PY - 2011/12

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N2 - Translational prostate cancer research is hampered by long intervals from diagnosis to patient progression and difficulty in obtaining cancer tissue for investigation. As such, it is imperative to utilise aging formalin-fixed paraffin-embedded (FFPE) tissue samples from the pathology archive with linked patient outcome data to allow current day research. This study aimed to assess the adequacy and quantity of mRNA extracted from formalin fixed paraffin embedded (FFPE) prostate tissue, including prostate biopsies, up to 15 years old. The decay of mRNA over time and under differing storage conditions was also assessed.

AB - Translational prostate cancer research is hampered by long intervals from diagnosis to patient progression and difficulty in obtaining cancer tissue for investigation. As such, it is imperative to utilise aging formalin-fixed paraffin-embedded (FFPE) tissue samples from the pathology archive with linked patient outcome data to allow current day research. This study aimed to assess the adequacy and quantity of mRNA extracted from formalin fixed paraffin embedded (FFPE) prostate tissue, including prostate biopsies, up to 15 years old. The decay of mRNA over time and under differing storage conditions was also assessed.

KW - Analysis of Variance

KW - Biopsy

KW - Formaldehyde

KW - Humans

KW - Male

KW - Paraffin Embedding

KW - Prostate

KW - Prostatic Hyperplasia

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Specimen Handling

KW - Temperature

KW - Time Factors

U2 - 10.1007/s11255-011-9948-3

DO - 10.1007/s11255-011-9948-3

M3 - Article

C2 - 21448682

SN - 1573-2584

VL - 43

SP - 961

EP - 967

JO - International urology and nephrology

JF - International urology and nephrology

IS - 4

ER -

Utilizing mRNA extracted from small, archival formalin-fixed paraffin-embedded prostate samples for translational research: assessment of the effect of increasing sample age and storage temperature

Abstract

Keywords / Materials (for Non-textual outputs)

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