Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

Kristel Sepp, Martin Lee, Marie T. J. Bluntzer, G. Vignir Helgason, Alison N. Hulme, Valerie G. Brunton

Research output: Contribution to journalArticlepeer-review

Abstract

Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.
Original languageEnglish
Pages (from-to)2028–2034
Number of pages7
JournalJournal of Medicinal Chemistry
Volume63
Issue number5
Early online date12 Dec 2019
DOIs
Publication statusE-pub ahead of print - 12 Dec 2019

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