Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

Kristel Sepp; Martin Lee; Marie T. J. Bluntzer; G. Vignir Helgason; Alison N. Hulme; Valerie G. Brunton

doi:10.1021/acs.jmedchem.9b01546

Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

Kristel Sepp, Martin Lee, Marie T. J. Bluntzer, G. Vignir Helgason, Alison N. Hulme, Valerie G. Brunton

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

Original language	English
Pages (from-to)	2028–2034
Number of pages	7
Journal	Journal of Medicinal Chemistry
Volume	63
Issue number	5
Early online date	12 Dec 2019
DOIs	https://doi.org/10.1021/acs.jmedchem.9b01546
Publication status	E-pub ahead of print - 12 Dec 2019

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10.1021/acs.jmedchem.9b01546

Utilizing stimulated Raman scattering microscopy to study intracellular distribution of ...Accepted author manuscript, 590 KB
Utilizing stimulated Raman scattering microscopy to study intracellular distribution ...Final published version, 4.92 MBLicence: Creative Commons: Attribution Non-Commercial (CC-BY-NC)

Cancer Research UK Edinburgh Centre Award 2017/2018
Frame, M. & Tomlinson, I.
UK-based charities
1/04/17 → 31/03/22
Project: Research
A HUB FOR ENGINEERING AND EXPLOITING THE STEM CELL NICHE
Forbes, S.
MRC
1/11/13 → 31/03/18
Project: Research
Invasion and metastasis; understanding and targeting an adhesion protein network - Programme Grant Renewal
Frame, M.
UK-based charities
1/05/13 → 31/08/18
Project: Research

Val Brunton
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of Cancer Therapeutics
- Edinburgh Cancer Research Centre
Person: Academic: Research Active
Alison Hulme
- School of Chemistry - Personal Chair of Synthesis and Chemical Biology
- EaStCHEM
Person: Academic: Research Active

Cite this

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title = "Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells",

abstract = "Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.",

author = "Kristel Sepp and Martin Lee and Bluntzer, {Marie T. J.} and Helgason, {G. Vignir} and Hulme, {Alison N.} and Brunton, {Valerie G.}",

year = "2019",

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doi = "10.1021/acs.jmedchem.9b01546",

language = "English",

volume = "63",

pages = "2028–2034",

journal = "Journal of Medicinal Chemistry",

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publisher = "American Chemical Society",

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T1 - Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

AU - Sepp, Kristel

AU - Lee, Martin

AU - Bluntzer, Marie T. J.

AU - Helgason, G. Vignir

AU - Hulme, Alison N.

AU - Brunton, Valerie G.

PY - 2019/12/12

Y1 - 2019/12/12

N2 - Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

AB - Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

U2 - 10.1021/acs.jmedchem.9b01546

DO - 10.1021/acs.jmedchem.9b01546

M3 - Article

SN - 0022-2623

VL - 63

SP - 2028

EP - 2034

JO - Journal of Medicinal Chemistry

JF - Journal of Medicinal Chemistry

IS - 5

ER -

Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

Abstract / Description of output

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Projects

Cancer Research UK Edinburgh Centre Award 2017/2018

A HUB FOR ENGINEERING AND EXPLOITING THE STEM CELL NICHE

Invasion and metastasis; understanding and targeting an adhesion protein network - Programme Grant Renewal

Profiles

Val Brunton

Alison Hulme

Cite this