Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen

L Vervelde*, N Bakker, FNJ Kooyman, AWCA Cornelissen, CMC Bank, AK Nyame, RD Cummings, Irma van Die

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Lambs respond to vaccination against bacteria and viruses but have a poor immunological response to nematodes. Here we report that they are protected against the parasitic nematode Haemonchus contortus after vaccination with excretory/secretory (ES) glycoproteins using Alhydrogel as an adjuvant. Lambs immunized with ES in Alhydrogel and challenged with 300 L3 larvae/kg body weight had a reduction in cumulative egg output of 89% and an increased percentage protection of 54% compared with the adjuvant control group. Compared to the adjuvant dimethyl dioctadecyl ammonium bromide, Alhydrogel induced earlier onset and significantly higher ES-specific IgG, IgA, and IgE antibody responses. In all vaccinated groups a substantial proportion of the antibody response was directed against glycan epitopes, irrespective of the adjuvant used. In lambs vaccinated with ES in Alhydrogel but not in any other group a significant increase was found in antibody levels against the GalNAcbeta1,4(Fucalpha1,3)GlcNAc (fucosylated LacdiNAc, LDNF) antigen, a carbohydrate antigen that is also involved in the host defense against the human parasite Schistosoma mansoni. In lambs the LDNF-specific response increased from the first immunization onward and was significantly higher in protected lambs. In addition, an isotype switch from LDNF-specific IgM to IgG was induced that correlated with protection. These data demonstrate that hyporesponsiveness of lambs to H. contortus can be overcome by vaccination with ES glycoproteins in a strong T-helper 2 type response-inducing aluminum adjuvant. This combination generated high and specific antiglycan antibody responses that may contribute to the vaccination-induced protection.

Original languageEnglish
Pages (from-to)795-804
Number of pages10
JournalGlycobiology
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 2003

Keywords

  • fucosylated LacdiNAc
  • MANSONI EGG ANTIGENS
  • excretory-secretory products
  • TRICHINELLA-SPIRALIS
  • Haemonchus
  • FUCOSYLATED LACDINAC
  • SCHISTOSOMA-MANSONI
  • INFECTED MICE
  • N-glycans
  • carbohydrate-specific antibody response
  • IMMUNE-RESPONSES
  • LEWIS-X
  • EXPRESSION
  • GLYCOPROTEINS
  • SHEEP

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