Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI

David M L Lilburn, Theodore Hughes-Riley, Joseph S Six, Karl F Stupic, Dominick E Shaw, Galina E Pavlovskaya, Thomas Meersmann

Research output: Contribution to journalArticlepeer-review

Abstract

Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) (129)Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp (129)Xe. Straightforward hp (129)Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp (129)Xe probe volumes during the inhalation cycle. Hp (129)Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies.

Original languageEnglish
Pages (from-to)e73468
JournalPLoS ONE
Volume8
Issue number8
DOIs
Publication statusPublished - 2013

Keywords

  • Animals
  • Dissection
  • Guinea Pigs
  • Humans
  • Inhalation
  • Lung
  • Magnetic Resonance Imaging
  • Male
  • Pulmonary Ventilation
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Residual Volume
  • Signal Processing, Computer-Assisted
  • Time Factors
  • Xenon
  • Xenon Isotopes

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