Validation of the isolation and quantification of kidney enriched miRNAs for use as biomarkers

Jack W. Sharkey, Daniel J. Antoine*, B. Kevin Park

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Context: MiRNAs have been reported to represent sensitive and translational biomarkers of organ injury

Objective: To validate the methodologies for the isolation and quantification of a miRNAs from none-invasive biofluids

Methods: Commercially available miRNA isolation kits and qPCR was utilised for determination of analyte sensitivity, stability, recovery, and precision

Results: qPCR was highly precise and sensitive for endogenous miRNA quantification (miR-194, LLQ; 0.1 pM). Intra and inter-assay variation remained low (

Conclusions: Translational endogenous miRNAs reflective of renal injury represent stable analytes with a large dynamic range that can be quantified easily in most laboratories

Original languageEnglish
Pages (from-to)231-239
Number of pages9
JournalBiomarkers
Volume17
Issue number3
DOIs
Publication statusPublished - May 2012

Keywords / Materials (for Non-textual outputs)

  • Nephrotoxicity
  • renal
  • biomarker
  • exosome
  • urine
  • INDUCED LIVER-INJURY
  • CIRCULATING MICRORNAS
  • EXPRESSION
  • STABILITY
  • MARKERS
  • SERUM
  • RNA

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