Abstract / Description of output
Context: MiRNAs have been reported to represent sensitive and translational biomarkers of organ injury
Objective: To validate the methodologies for the isolation and quantification of a miRNAs from none-invasive biofluids
Methods: Commercially available miRNA isolation kits and qPCR was utilised for determination of analyte sensitivity, stability, recovery, and precision
Results: qPCR was highly precise and sensitive for endogenous miRNA quantification (miR-194, LLQ; 0.1 pM). Intra and inter-assay variation remained low (
Conclusions: Translational endogenous miRNAs reflective of renal injury represent stable analytes with a large dynamic range that can be quantified easily in most laboratories
Original language | English |
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Pages (from-to) | 231-239 |
Number of pages | 9 |
Journal | Biomarkers |
Volume | 17 |
Issue number | 3 |
DOIs | |
Publication status | Published - May 2012 |
Keywords / Materials (for Non-textual outputs)
- Nephrotoxicity
- renal
- biomarker
- exosome
- urine
- INDUCED LIVER-INJURY
- CIRCULATING MICRORNAS
- EXPRESSION
- STABILITY
- MARKERS
- SERUM
- RNA