Projects per year
Abstract
Two different forms of clinical paratuberculosis in sheep are recognised, related to the level of bacterial colonization. Paucibacillary lesions are largely composed of lymphocytes with few bacteria, and multibacillary pathology is characterized by heavily-infected macrophages. Analysis of cytokine transcripts has shown that inflammatory Th1/Th17 T cells are associated with development of paucibacillary pathology and Th2 cytokines are correlated with multibacillary disease. The master regulator T cell transcription factors TBX21, GATA3, RORC2 and RORA are critical for the development of these T cell subsets. Sequence variations of the transcription factors have also been implicated in the distinct disease forms of human mycobacterial and gastrointestinal inflammatory diseases. Relative RT-qPCR was used to compare expression levels of each transcript variant of the master regulators in the ileo-caecal lymph nodes of uninfected controls and sheep with defined paucibacillary and multibacillary pathology. Low levels of GATA3 in multibacillary sheep failed to confirm that multibacillary paratuberculosis is caused simply by a Th2 immune response. However, high levels of TBX21, RORC2 and RORC2v1 highlights the role of Th1 and Th17 activation in paucibacillary disease. Increased RORAv1 levels in paucibacillary tissue suggests a role for RORα in Th17 development in sheep; while elevated levels of RORAv4 hints that this variant might inhibit RORα function and depress Th17 development in multibacillary sheep.
Original language | English |
---|---|
Article number | 83 |
Journal | Veterinary Research |
Volume | 47 |
DOIs | |
Publication status | Published - 17 Aug 2016 |
Fingerprint
Dive into the research topics of 'Variations in T cell transcription factor gene structure and expression associated with the two disease forms of sheep paratuberculosis'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Innate immunity and endemic diseases in livestock species
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
1/04/12 → 31/03/17
Project: Research
Datasets
-
John_Hopkins_Paratuberculosis_Archive
Simpson, C. (Depositor) & Hopkins, J. (Creator), Edinburgh DataVault, 2017
Dataset
-
John_Hopkins_Tcircumcincta_Archive
Hopkins, J. (Creator), Simpson, C. (Depositor), Gossner, A. (Data Collector) & Pemberton, J. (Data Collector), Edinburgh DataVault, 2017
DOI: 10.7488/a2e7c6ae-0502-4a56-bddd-9325fbed7637
Dataset