Video rate spectral fluorescence lifetime imaging with a 512x16 pixel line sensor

Ahmet Erdogan, Neil Finlayson, Gareth Williams, Elvira Bohl, Robert Henderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate a 512 x 16 single photon avalanche diode (SPAD) line sensor with per-pixel on-chip histogramming for video rate spectral fluorescence lifetime imaging. On-chip histogramming provides 32-bin histograms per pixel with 11bit/bin dynamic range. In addition, bin widths in time can be programmed from 51.20 ps to 6.55 ns, providing a histogram range from 1.64 ns to 209.72 ns to suit a wide range of fluorescence decays. In this paper we study the lifetime accuracy vs image frame rate trade-offs by varying the number of histogram bins while carefully adjusting the bin widths for maximum bin counts.
Original languageEnglish
Title of host publicationSPIE Photonics West Paper 10889-22
Subtitle of host publicationHigh-Speed Biomedical Imaging and Spectroscopy IV
Publication statusPublished - 2019

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