We demonstrate a 512 x 16 single photon avalanche diode (SPAD) line sensor with per-pixel on-chip histogramming for video rate spectral fluorescence lifetime imaging. On-chip histogramming provides 32-bin histograms per pixel with 11bit/bin dynamic range. In addition, bin widths in time can be programmed from 51.20 ps to 6.55 ns, providing a histogram range from 1.64 ns to 209.72 ns to suit a wide range of fluorescence decays. In this paper we study the lifetime accuracy vs image frame rate trade-offs by varying the number of histogram bins while carefully adjusting the bin widths for maximum bin counts.
|Title of host publication||SPIE Photonics West Paper 10889-22|
|Subtitle of host publication||High-Speed Biomedical Imaging and Spectroscopy IV|
|Publication status||Published - 2019|