Virome of US bovine calf serum

Mohammadreza Sadeghi; Beatrix Kapusinszky; Danielle M. Yugo; Tung Gia Phan; Xutao Deng; Isis Kanevsky; Tanja Opriessnig; Amelia R. Woolums; David J. Hurley; Xiang-jin Meng; Eric Delwart

doi:10.1016/j.biologicals.2016.12.009

Virome of US bovine calf serum

Mohammadreza Sadeghi, Beatrix Kapusinszky, Danielle M. Yugo, Tung Gia Phan, Xutao Deng, Isis Kanevsky, Tanja Opriessnig, Amelia R. Woolums, David J. Hurley, Xiang-jin Meng, Eric Delwart

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

Original language	English
Journal	Biologicals
Early online date	15 Jan 2017
DOIs	https://doi.org/10.1016/j.biologicals.2016.12.009
Publication status	E-pub ahead of print - 15 Jan 2017

Keywords / Materials (for Non-textual outputs)

Metagenomics
Parvovirus
Bosavirus
Cell culture

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10.1016/j.biologicals.2016.12.009

Virome of US bovine calf serumAccepted author manuscript, 425 KBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

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title = "Virome of US bovine calf serum",

abstract = "Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.",

keywords = "Metagenomics, Parvovirus, Bosavirus, Cell culture",

author = "Mohammadreza Sadeghi and Beatrix Kapusinszky and Yugo, {Danielle M.} and Phan, {Tung Gia} and Xutao Deng and Isis Kanevsky and Tanja Opriessnig and Woolums, {Amelia R.} and Hurley, {David J.} and Xiang-jin Meng and Eric Delwart",

year = "2017",

month = jan,

day = "15",

doi = "10.1016/j.biologicals.2016.12.009",

language = "English",

journal = "Biologicals",

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T1 - Virome of US bovine calf serum

AU - Sadeghi, Mohammadreza

AU - Kapusinszky, Beatrix

AU - Yugo, Danielle M.

AU - Phan, Tung Gia

AU - Deng, Xutao

AU - Kanevsky, Isis

AU - Opriessnig, Tanja

AU - Woolums, Amelia R.

AU - Hurley, David J.

AU - Meng, Xiang-jin

AU - Delwart, Eric

PY - 2017/1/15

Y1 - 2017/1/15

N2 - Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

AB - Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

KW - Metagenomics

KW - Parvovirus

KW - Bosavirus

KW - Cell culture

U2 - 10.1016/j.biologicals.2016.12.009

DO - 10.1016/j.biologicals.2016.12.009

M3 - Article

SN - 1045-1056

JO - Biologicals

JF - Biologicals

ER -

Virome of US bovine calf serum

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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