Visualisation of chicken macrophages using transgenic reporter genes:  insights into the development of the avian macrophage lineage

Adam Balic; Carla Garcia-Morales; Lonneke Vervelde; Hazel Gilhooley; Adrian Sherman; Valerie Garceau; Maria Gutowska; Dave Burt; Pete Kaiser; David Hume; Helen Sang

doi:10.1242/dev.105593

Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage

Adam Balic, Carla Garcia-Morales, Lonneke Vervelde, Hazel Gilhooley, Adrian Sherman, Valerie Garceau, Maria Gutowska, Dave Burt, Pete Kaiser, David Hume, Helen Sang

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.

Original language	English
Pages (from-to)	3255-3265
Journal	Development
Volume	141
Issue number	16
DOIs	https://doi.org/10.1242/dev.105593
Publication status	Published - 6 Aug 2014

Access to Document

10.1242/dev.105593

PDF - Visualisation of chicken macrophages using transgenic reporter genes
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Final published version, 1.55 MB

http://dev.biologists.org/content/141/16/3255

Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
BBSRC
1/04/12 → 31/03/17
Project: Research
CSF1 IL34 and the function of macrophages in avain development
Hume, D. (Principal Investigator), Kaiser, P. (Co-investigator) & Sang, H. (Co-investigator)
BBSRC
1/06/10 → 11/08/13
Project: Research

Genome engineering in the chicken
Sang, H. (Speaker)
28 Mar 2016 → 31 Mar 2016
Activity: Academic talk or presentation types › Invited talk

Cite this

@article{6fc17917c28f48de8bcf72281b01411c,

title = "Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage",

abstract = "We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.",

author = "Adam Balic and Carla Garcia-Morales and Lonneke Vervelde and Hazel Gilhooley and Adrian Sherman and Valerie Garceau and Maria Gutowska and Dave Burt and Pete Kaiser and David Hume and Helen Sang",

year = "2014",

month = aug,

day = "6",

doi = "10.1242/dev.105593",

language = "English",

volume = "141",

pages = "3255--3265",

journal = "Development",

issn = "0950-1991",

publisher = "Company of Biologists Ltd",

number = "16",

}

TY - JOUR

T1 - Visualisation of chicken macrophages using transgenic reporter genes

T2 - insights into the development of the avian macrophage lineage

AU - Balic, Adam

AU - Garcia-Morales, Carla

AU - Vervelde, Lonneke

AU - Gilhooley, Hazel

AU - Sherman, Adrian

AU - Garceau, Valerie

AU - Gutowska, Maria

AU - Burt, Dave

AU - Kaiser, Pete

AU - Hume, David

AU - Sang, Helen

PY - 2014/8/6

Y1 - 2014/8/6

N2 - We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.

AB - We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.

U2 - 10.1242/dev.105593

DO - 10.1242/dev.105593

M3 - Article

SN - 0950-1991

VL - 141

SP - 3255

EP - 3265

JO - Development

JF - Development

IS - 16

ER -

Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage

Abstract

Access to Document

Fingerprint

Projects

Innate immunity and endemic diseases in livestock species

CSF1 IL34 and the function of macrophages in avain development

Activities

Genome engineering in the chicken

Cite this