Projects per year
Abstract / Description of output
We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.
Original language | English |
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Pages (from-to) | 3255-3265 |
Journal | Development |
Volume | 141 |
Issue number | 16 |
DOIs | |
Publication status | Published - 6 Aug 2014 |
Fingerprint
Dive into the research topics of 'Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage'. Together they form a unique fingerprint.Projects
- 2 Finished
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Innate immunity and endemic diseases in livestock species
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
1/04/12 → 31/03/17
Project: Research
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CSF1 IL34 and the function of macrophages in avain development
Hume, D., Kaiser, P. & Sang, H.
1/06/10 → 11/08/13
Project: Research
Activities
- 1 Invited talk
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Genome engineering in the chicken
Helen Sang (Speaker)
28 Mar 2016 → 31 Mar 2016Activity: Academic talk or presentation types › Invited talk
Profiles
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Lonneke Vervelde
- Royal (Dick) School of Veterinary Studies - Personal Chair of Veterinary Immunology &Infectious Diseases
Person: Academic: Research Active